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Engineering Isoprenoid Biosynthesis in Artemisia annua L. for the Production of Taxadiene: A Key Intermediate of Taxol
Joint Authors
Li, Meiya
Jiang, Fusheng
Yu, Xiangli
Miao, Zhiqi
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Taxadiene is the first committed precursor to paclitaxel, marketed as Taxol, arguably the most important anticancer agent against ovarian and breast cancer.
In Taxus, taxadiene is directly synthesized from geranylgeranyl diphosphate (GGPP) that is the common precursor for diterpenoids and is found in most plants and microbes.
In this study, Artemisia annua L., a Chinese medicinal herb that grows fast and is rich in terpenoids, was used as a genetic engineering host to produce taxadiene.
The TXS (taxadiene synthase) gene, cloned from Taxus and inserted into pCAMBIA1304, was transformed into Artemisia annua L.
using the Agrobacterium tumefaciens-mediated method.
Thirty independent transgenic plants were obtained, and GC-MS analysis was used to confirm that taxadiene was produced and accumulated up to 129.7 μg/g dry mass.
However, the high expression of TXS did not affect plant growth or photosynthesis in transgenic Artemisia annua L.
It is notable that artemisinin is produced and stored in leaves and most taxadiene accumulated in the stem of transgenic Artemisia annua L., suggesting a new way to produce two important compounds in one transgenic plant: leaves for artemisinin and stem for taxadiene.
Overall, this study demonstrates that genetic engineering of the taxane biosynthetic pathway in Artemisia annua L.
for the production of taxadiene is feasible.
American Psychological Association (APA)
Li, Meiya& Jiang, Fusheng& Yu, Xiangli& Miao, Zhiqi. 2015. Engineering Isoprenoid Biosynthesis in Artemisia annua L. for the Production of Taxadiene: A Key Intermediate of Taxol. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055729
Modern Language Association (MLA)
Li, Meiya…[et al.]. Engineering Isoprenoid Biosynthesis in Artemisia annua L. for the Production of Taxadiene: A Key Intermediate of Taxol. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055729
American Medical Association (AMA)
Li, Meiya& Jiang, Fusheng& Yu, Xiangli& Miao, Zhiqi. Engineering Isoprenoid Biosynthesis in Artemisia annua L. for the Production of Taxadiene: A Key Intermediate of Taxol. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055729
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055729