The Use of an IL-1 Receptor Antagonist Peptide to Control Inflammation in the Treatment of Corneal Limbal Epithelial Stem Cell Deficiency
Joint Authors
Guildford, A.
Fok, E.
Sandeman, S. R.
Martin, Y. H.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-01
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Corneal limbal stem cell deficiency (LSCD) may be treated using ex vivo limbal epithelial stem cells (LESCs) derived from cadaveric donor tissue.
However, continuing challenges exist around tissue availability, inflammation, and transplant rejection.
Lipopolysaccharide (LPS) or recombinant human IL-1β stimulated primary human keratocyte and LESC models were used to investigate the anti-inflammatory properties of a short chain, IL-1 receptor antagonist peptide for use in LESC sheet growth to control inflammation.
The peptide was characterized using mass spectroscopy and high performance liquid chromatography.
Peptide cytotoxicity, patterns of cell cytokine expression in response to LPS or IL-1β stimulation, and peptide suppression of this response were investigated by MTS/LDH assays, ELISA, and q-PCR.
Cell differences in LPS stimulated toll-like receptor 4 expression were investigated using immunocytochemistry.
A significant reduction in rIL-1β stimulated inflammatory cytokine production occurred following LESC and keratocyte incubation with anti-inflammatory peptide and in LPS stimulated IL-6 and IL-8 production following keratocyte incubation with peptide (1 mg/mL) P<0.05.
LESCs produced no cytokine response to LPS stimulation and showed no TLR4 expression.
The peptide supported LESC growth when adhered to a silicone hydrogel contact lens indicating potential use in improved LESC grafting through suppression of inflammation.
American Psychological Association (APA)
Fok, E.& Sandeman, S. R.& Guildford, A.& Martin, Y. H.. 2015. The Use of an IL-1 Receptor Antagonist Peptide to Control Inflammation in the Treatment of Corneal Limbal Epithelial Stem Cell Deficiency. BioMed Research International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1055784
Modern Language Association (MLA)
Fok, E.…[et al.]. The Use of an IL-1 Receptor Antagonist Peptide to Control Inflammation in the Treatment of Corneal Limbal Epithelial Stem Cell Deficiency. BioMed Research International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1055784
American Medical Association (AMA)
Fok, E.& Sandeman, S. R.& Guildford, A.& Martin, Y. H.. The Use of an IL-1 Receptor Antagonist Peptide to Control Inflammation in the Treatment of Corneal Limbal Epithelial Stem Cell Deficiency. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1055784
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055784