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Acoustic-Frequency Vibratory Stimulation Regulates the Balance between Osteogenesis and Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells
Joint Authors
He, Fan
Luo, Zong-Ping
Chen, Xi
Zhong, Dong-Yan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Osteoporosis can be associated with the disordered balance between osteogenesis and adipogenesis of bone marrow-derived mesenchymal stem cells (BM-MSCs).
Although low-frequency mechanical vibration has been demonstrated to promote osteogenesis, little is known about the influence of acoustic-frequency vibratory stimulation (AFVS).
BM-MSCs were subjected to AFVS at frequencies of 0, 30, 400, and 800 Hz and induced toward osteogenic or adipogenic-specific lineage.
Extracellular matrix mineralization was determined by Alizarin Red S staining and lipid accumulation was assessed by Oil Red O staining.
Transcript levels of osteogenic and adipogenic marker genes were evaluated by real-time reverse transcription-polymerase chain reaction.
Cell proliferation of BM-MSCs was promoted following exposure to AFVS at 800 Hz.
Vibration at 800 Hz induced the highest level of calcium deposition and significantly increased mRNA expression of COL1A1, ALP, RUNX2, and SPP1.
The 800 Hz group downregulated lipid accumulation and levels of adipogenic genes, including FABP4, CEBPA, PPARG, and LEP, while vibration at 30 Hz supported adipogenesis.
BM-MSCs showed a frequency-dependent response to acoustic vibration.
AFVS at 800 Hz was the most favorable for osteogenic differentiation and simultaneously suppressed adipogenesis.
Thus, acoustic vibration could potentially become a novel means to prevent and treat osteoporosis.
American Psychological Association (APA)
Chen, Xi& He, Fan& Zhong, Dong-Yan& Luo, Zong-Ping. 2015. Acoustic-Frequency Vibratory Stimulation Regulates the Balance between Osteogenesis and Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055881
Modern Language Association (MLA)
Chen, Xi…[et al.]. Acoustic-Frequency Vibratory Stimulation Regulates the Balance between Osteogenesis and Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055881
American Medical Association (AMA)
Chen, Xi& He, Fan& Zhong, Dong-Yan& Luo, Zong-Ping. Acoustic-Frequency Vibratory Stimulation Regulates the Balance between Osteogenesis and Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055881
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055881