A Whole-Genome Microarray Study of Arabidopsis thaliana Semisolid Callus Cultures Exposed to Microgravity and Nonmicrogravity Related Spaceflight Conditions for 5 Days on Board of Shenzhou 8
Joint Authors
Fengler, Svenja
Spirer, Ina
Neef, Maren
Ecke, Margret
Nieselt, Kay
Hampp, Rüdiger
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-13
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The Simbox mission was the first joint space project between Germany and China in November 2011.
Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8.
The related ground experiment was conducted under similar conditions.
The use of an in-flight centrifuge provided a 1 g gravitational field in space.
The cells were metabolically quenched after 5 days via RNAlater injection.
The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis.
The results show a major impact of nonmicrogravity related spaceflight conditions.
Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses.
In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity.
These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production.
American Psychological Association (APA)
Fengler, Svenja& Spirer, Ina& Neef, Maren& Ecke, Margret& Nieselt, Kay& Hampp, Rüdiger. 2015. A Whole-Genome Microarray Study of Arabidopsis thaliana Semisolid Callus Cultures Exposed to Microgravity and Nonmicrogravity Related Spaceflight Conditions for 5 Days on Board of Shenzhou 8. BioMed Research International،Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1055900
Modern Language Association (MLA)
Fengler, Svenja…[et al.]. A Whole-Genome Microarray Study of Arabidopsis thaliana Semisolid Callus Cultures Exposed to Microgravity and Nonmicrogravity Related Spaceflight Conditions for 5 Days on Board of Shenzhou 8. BioMed Research International No. 2015 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1055900
American Medical Association (AMA)
Fengler, Svenja& Spirer, Ina& Neef, Maren& Ecke, Margret& Nieselt, Kay& Hampp, Rüdiger. A Whole-Genome Microarray Study of Arabidopsis thaliana Semisolid Callus Cultures Exposed to Microgravity and Nonmicrogravity Related Spaceflight Conditions for 5 Days on Board of Shenzhou 8. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1055900
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055900