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Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting
Joint Authors
McAllister, Tim A.
Badhan, Ajay
Wang, Yu-Xi
Gruninger, Robert
Patton, Donald
Powlowski, Justin
Tsang, Adrian
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Identification of recalcitrant factors that limit digestion of forages and the development of enzymatic approaches that improve hydrolysis could play a key role in improving the efficiency of meat and milk production in ruminants.
Enzyme fingerprinting of barley silage fed to heifers and total tract indigestible fibre residue (TIFR) collected from feces was used to identify cell wall components resistant to total tract digestion.
Enzyme fingerprinting results identified acetyl xylan esterases as key to the enhanced ruminal digestion.
FTIR analysis also suggested cross-link cell wall polymers as principal components of indigested fiber residues in feces.
Based on structural information from enzymatic fingerprinting and FTIR, enzyme pretreatment to enhance glucose yield from barley straw and alfalfa hay upon exposure to mixed rumen-enzymes was developed.
Prehydrolysis effects of recombinant fungal fibrolytic hydrolases were analyzed using microassay in combination with statistical experimental design.
Recombinant hemicellulases and auxiliary enzymes initiated degradation of plant structural polysaccharides upon application and improved the in vitro saccharification of alfalfa and barley straw by mixed rumen enzymes.
The validation results showed that microassay in combination with statistical experimental design can be successfully used to predict effective enzyme pretreatments that can enhance plant cell wall digestion by mixed rumen enzymes.
American Psychological Association (APA)
Badhan, Ajay& Wang, Yu-Xi& Gruninger, Robert& Patton, Donald& Powlowski, Justin& Tsang, Adrian…[et al.]. 2015. Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1055922
Modern Language Association (MLA)
Badhan, Ajay…[et al.]. Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1055922
American Medical Association (AMA)
Badhan, Ajay& Wang, Yu-Xi& Gruninger, Robert& Patton, Donald& Powlowski, Justin& Tsang, Adrian…[et al.]. Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1055922
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055922