![](/images/graphics-bg.png)
How to Isolate a Plant’s Hypomethylome in One Shot
Joint Authors
Wischnitzki, Elisabeth
Sehr, Eva Maria
Hansel-Hohl, Karin
Berenyi, Maria
Burg, Kornel
Fluch, Silvia
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Genome assembly remains a challenge for large and/or complex plant genomes due to their abundant repetitive regions resulting in studies focusing on gene space instead of the whole genome.
Thus, DNA enrichment strategies facilitate the assembly by increasing the coverage and simultaneously reducing the complexity of the whole genome.
In this paper we provide an easy, fast, and cost-effective variant of MRE-seq to obtain a plant’s hypomethylome by an optimized methyl filtration protocol followed by next generation sequencing.
The method is demonstrated on three plant species with knowingly large and/or complex (polyploid) genomes: Oryza sativa, Picea abies, and Crocus sativus.
The identified hypomethylomes show clear enrichment for genes and their flanking regions and clear reduction of transposable elements.
Additionally, genomic sequences around genes are captured including regulatory elements in introns and up- and downstream flanks.
High similarity of the results obtained by a de novo assembly approach with a reference based mapping in rice supports the applicability for studying and understanding the genomes of nonmodel organisms.
Hence we show the high potential of MRE-seq in a wide range of scenarios for the direct analysis of methylation differences, for example, between ecotypes, individuals, within or across species harbouring large, and complex genomes.
American Psychological Association (APA)
Wischnitzki, Elisabeth& Sehr, Eva Maria& Hansel-Hohl, Karin& Berenyi, Maria& Burg, Kornel& Fluch, Silvia. 2015. How to Isolate a Plant’s Hypomethylome in One Shot. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055952
Modern Language Association (MLA)
Wischnitzki, Elisabeth…[et al.]. How to Isolate a Plant’s Hypomethylome in One Shot. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1055952
American Medical Association (AMA)
Wischnitzki, Elisabeth& Sehr, Eva Maria& Hansel-Hohl, Karin& Berenyi, Maria& Burg, Kornel& Fluch, Silvia. How to Isolate a Plant’s Hypomethylome in One Shot. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055952
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055952