Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon- γ Signaling
Joint Authors
Shan, Yuqun
Lu, Xingxiao
Han, Yingnan
Li, Xinpeng
Wang, Xiao
Shao, Chunhong
Wang, Lixiang
Liu, Zhifang
Tang, Wei
Sun, Yundong
Jia, Jihui
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-06
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Outer membrane proteins (OMPs) can induce an immune response.
Omp18 (HP1125) of H.
pylori is a powerful antigen that can induce significant interferon-γ (IFN-γ) levels.
Previous studies have suggested that IFN-γ plays an important role in H.
pylori clearance.
However, H.
pylori has multiple mechanisms to avoid host immune surveillance for persistent colonization.
We generated an omp18 mutant (H.
pylori 26695 and H.
pylori SS1) strain to examine whether Omp18 interacts with IFN-γ and is involved in H.
pylori colonization.
qRT-PCR revealed that IFN-γ induced Omp18 expression.
qRT-PCR and western blot analysis revealed reduced expressions of virulence factors CagA and NapA in H.
pylori 26695 with IFN-γ treatment, but they were induced in the Δomp18 strain.
In C57BL/6 mice infected with H.
pylori SS1 and the Δomp18 strain, the Δomp18 strain conferred defective colonization and activated a stronger inflammatory response.
Signal transducer phosphorylation and transcription 1 (STAT1) activator was downregulated by the wild-type strain but not the Δomp18 strain in IFN-γ-treated macrophages.
Furthermore, Δomp18 strain survival rates were poor in macrophages compared to the wild-type strain.
We concluded that H.
pylori Omp18 has an important function influencing IFN-γ-mediated immune response to participate in persistent colonization.
American Psychological Association (APA)
Shan, Yuqun& Lu, Xingxiao& Han, Yingnan& Li, Xinpeng& Wang, Xiao& Shao, Chunhong…[et al.]. 2015. Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon- γ Signaling. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055954
Modern Language Association (MLA)
Shan, Yuqun…[et al.]. Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon- γ Signaling. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1055954
American Medical Association (AMA)
Shan, Yuqun& Lu, Xingxiao& Han, Yingnan& Li, Xinpeng& Wang, Xiao& Shao, Chunhong…[et al.]. Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon- γ Signaling. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055954
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055954