Helicobacter pylori Outer Membrane Protein 18 (Hp1125)‎ Is Involved in Persistent Colonization by Evading Interferon- γ Signaling

Joint Authors

Shan, Yuqun
Lu, Xingxiao
Han, Yingnan
Li, Xinpeng
Wang, Xiao
Shao, Chunhong
Wang, Lixiang
Liu, Zhifang
Tang, Wei
Sun, Yundong
Jia, Jihui

Source

BioMed Research International

Issue

Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2015-04-06

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Medicine

Abstract EN

Outer membrane proteins (OMPs) can induce an immune response.

Omp18 (HP1125) of H.

pylori is a powerful antigen that can induce significant interferon-γ (IFN-γ) levels.

Previous studies have suggested that IFN-γ plays an important role in H.

pylori clearance.

However, H.

pylori has multiple mechanisms to avoid host immune surveillance for persistent colonization.

We generated an omp18 mutant (H.

pylori 26695 and H.

pylori SS1) strain to examine whether Omp18 interacts with IFN-γ and is involved in H.

pylori colonization.

qRT-PCR revealed that IFN-γ induced Omp18 expression.

qRT-PCR and western blot analysis revealed reduced expressions of virulence factors CagA and NapA in H.

pylori 26695 with IFN-γ treatment, but they were induced in the Δomp18 strain.

In C57BL/6 mice infected with H.

pylori SS1 and the Δomp18 strain, the Δomp18 strain conferred defective colonization and activated a stronger inflammatory response.

Signal transducer phosphorylation and transcription 1 (STAT1) activator was downregulated by the wild-type strain but not the Δomp18 strain in IFN-γ-treated macrophages.

Furthermore, Δomp18 strain survival rates were poor in macrophages compared to the wild-type strain.

We concluded that H.

pylori Omp18 has an important function influencing IFN-γ-mediated immune response to participate in persistent colonization.

American Psychological Association (APA)

Shan, Yuqun& Lu, Xingxiao& Han, Yingnan& Li, Xinpeng& Wang, Xiao& Shao, Chunhong…[et al.]. 2015. Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon- γ Signaling. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055954

Modern Language Association (MLA)

Shan, Yuqun…[et al.]. Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon- γ Signaling. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1055954

American Medical Association (AMA)

Shan, Yuqun& Lu, Xingxiao& Han, Yingnan& Li, Xinpeng& Wang, Xiao& Shao, Chunhong…[et al.]. Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon- γ Signaling. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055954

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1055954