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Mcl-1 Is a Novel Target of miR-26b That Is Associated with the Apoptosis Induced by TRAIL in HCC Cells
Joint Authors
Jiang, Chunlin
Long, Jianting
Liu, Baoxian
Xie, Xiaoyan
Kuang, Ming
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aim.
To investigate the role of miR-26b and Mcl-1 in TRAIL-inducing cell death in hepatocellular carcinoma.
Methods.
The expression of miR-26b and Mcl-1 in HCC was detected by RT-qPCR and western blot.
The regulation of Mcl-1 by miR-26b was determined by luciferase reporter assay.
MTT and flow cytometry were employed to detect the cell viability and apoptosis.
Results.
miR-26b is commonly downregulated in HCC cell lines compared with the LO2 cell line.
In contrast, the Mcl-1 expression is upregulated in HCC cell lines.
Bioinformatic analysis identified a putative target site in the Mcl-1 mRNA for miR-26b and luciferase reporter assay showed that miR-26b directly targeted the 3′-UTR (3′-Untranslated Regions) of Mcl-1 mRNA.
Transfection of miR-26b mimics suppressed Mcl-1 expression in HCC cells and sensitized the cancer cells to TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) cytotoxicity.
In addition, transfection of HCC cells with Mcl-1 expression plasmid abolished the sensitization effect of miR-26b to TRAIL-inducing apoptosis.
Conclusions.
Our study showed that miR-26b was a negative regulator of Mcl-1 gene and sensitized TRAIL-inducing apoptosis in HCC cells, suggesting that the miR-26b-Mcl-1 pathway might be a novel target for the treatment of HCC.
American Psychological Association (APA)
Jiang, Chunlin& Long, Jianting& Liu, Baoxian& Xie, Xiaoyan& Kuang, Ming. 2015. Mcl-1 Is a Novel Target of miR-26b That Is Associated with the Apoptosis Induced by TRAIL in HCC Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055962
Modern Language Association (MLA)
Jiang, Chunlin…[et al.]. Mcl-1 Is a Novel Target of miR-26b That Is Associated with the Apoptosis Induced by TRAIL in HCC Cells. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055962
American Medical Association (AMA)
Jiang, Chunlin& Long, Jianting& Liu, Baoxian& Xie, Xiaoyan& Kuang, Ming. Mcl-1 Is a Novel Target of miR-26b That Is Associated with the Apoptosis Induced by TRAIL in HCC Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055962
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055962