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The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix
Joint Authors
Mahara, Atsushi
Morimoto, Naoki
Shima, Kouji
Ogawa, Mami
Jinno, Chizuru
Kakudo, Natsuko
Kusumoto, Kenji
Fujisato, Toshia
Suzuki, Shigehiko
Yamaoka, Tetsuji
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We previously reported that high hydrostatic pressure (HHP) of 200 MPa for 10 minutes could induce cell killing.
In this study, we explored whether HHP at 200 MPa or HHP at lower pressure, in combination with hyposmotic distilled water (DW), could inactivate the skin, as well as cultured cells.
We investigated the inactivation of porcine skin samples 4 mm in diameter.
They were immersed in either a normal saline solution (NSS) or DW, and then were pressurized at 100 and 200 MPa for 5, 10, 30, or 60 min.
Next, we explored the inactivation of specimens punched out from the pressurized skin 10 × 2 cm in size.
The viability was evaluated using a WST-8 assay and an outgrowth culture.
The histology of specimens was analyzed histologically.
The mitochondrial activity was inactivated after the pressurization at 200 MPa in both experiments, and no outgrowth was observed after the pressurization at 200 MPa.
The arrangement and proportion of the dermal collagen fibers or the elastin fibers were not adversely affected after the pressurization at 200 MPa for up to 60 minutes.
This study showed that a HHP at 200 MPa for 10 min could inactivate the skin without damaging the dermal matrix.
American Psychological Association (APA)
Morimoto, Naoki& Mahara, Atsushi& Shima, Kouji& Ogawa, Mami& Jinno, Chizuru& Kakudo, Natsuko…[et al.]. 2015. The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056022
Modern Language Association (MLA)
Morimoto, Naoki…[et al.]. The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056022
American Medical Association (AMA)
Morimoto, Naoki& Mahara, Atsushi& Shima, Kouji& Ogawa, Mami& Jinno, Chizuru& Kakudo, Natsuko…[et al.]. The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056022
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056022