A Novel Rat Model of Vitamin D Deficiency: Safe and Rapid Induction of Vitamin D and Calcitriol Deficiency without Hyperparathyroidism
Joint Authors
Beelen, Robert H. J.
Ferrantelli, E.
Keuning, E. D.
Vila Cuenca, M.
Vervloet, Marc G.
Arcidiacono, Maria Vittoria
Dusso, Adriana S.
Stavenuiter, Andrea W. D.
ter Wee, Piet M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Vitamin D deficiency is associated with a range of clinical disorders.
To study the mechanisms involved and improve treatments, animal models are tremendously useful.
Current vitamin D deficient rat models have important practical limitations, including time requirements when using, exclusively, a vitamin D deficient diet.
More importantly, induction of hypovitaminosis D causes significant fluctuations in parathyroid hormone (PTH) and mineral levels, complicating the interpretation of study results.
To overcome these shortcomings, we report the successful induction of vitamin D deficiency within three weeks, with stable serum PTH and minerals levels, in Wistar rats.
We incorporated two additional manoeuvres compared to a conventional diet.
Firstly, the vitamin D depleted diet is calcium (Ca) enriched, to attenuate the development of secondary hyperparathyroidism.
Secondly, six intraperitoneal injections of paricalcitol during the first two weeks are given to induce the rapid degradation of circulating vitamin D metabolites.
After three weeks, serum 25-hydroxyvitamin D3 (25D) and 1,25-dihydroxyvitamin D3 (1,25D) levels had dropped below detection limits, with unchanged serum PTH, Ca, and phosphate (P) levels.
Therefore, this model provides a useful tool to examine the sole effect of hypovitaminosis D, in a wide range of research settings, without confounding changes in PTH, Ca, and P.
American Psychological Association (APA)
Stavenuiter, Andrea W. D.& Arcidiacono, Maria Vittoria& Ferrantelli, E.& Keuning, E. D.& Vila Cuenca, M.& ter Wee, Piet M.…[et al.]. 2015. A Novel Rat Model of Vitamin D Deficiency: Safe and Rapid Induction of Vitamin D and Calcitriol Deficiency without Hyperparathyroidism. BioMed Research International،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1056088
Modern Language Association (MLA)
Stavenuiter, Andrea W. D.…[et al.]. A Novel Rat Model of Vitamin D Deficiency: Safe and Rapid Induction of Vitamin D and Calcitriol Deficiency without Hyperparathyroidism. BioMed Research International No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1056088
American Medical Association (AMA)
Stavenuiter, Andrea W. D.& Arcidiacono, Maria Vittoria& Ferrantelli, E.& Keuning, E. D.& Vila Cuenca, M.& ter Wee, Piet M.…[et al.]. A Novel Rat Model of Vitamin D Deficiency: Safe and Rapid Induction of Vitamin D and Calcitriol Deficiency without Hyperparathyroidism. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1056088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056088