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Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells
Joint Authors
Cheng, Ying
Du, Li
Jiao, Hanwei
Zhu, Huapei
Xu, Kailian
Guo, Shiyu
Shi, Qiaoyun
Zhao, Tianjing
Pang, Feng
Jia, Xiaoxiao
Wang, Fengyang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Lipopolysaccharide (LPS) stimulates macrophages to release proinflammatory cytokines.
MicroRNAs (miRNAs) are short noncoding RNAs that are involved in inflammatory reaction.
Our previously study identified the downregulated expression of mmu-miR-27a-5p in RAW267.4 cells treated with LPS.
To dissect the mechanism that mmu-miR-27a-5p regulates target genes and affects proinflammatory cytokine secretion more clearly, based on previous bioinformatics prediction data, one of the potential target genes, MCPIP1 was observed to be upregulated with qRT-PCR and western blot.
Luciferase reporter assays were performed to further confirm in silico prediction and determine that MCPIP1 is the target of mmu-miR-27-5p.
The results suggested that mmu-miR-27a-5p directly targeted the 3′-UTR of MCPIP1 and the interaction between mmu-miR-27-5p and the 3′-UTR of MCPIP1 is sequence-specific.
MCPIP1 overexpression decreased the secretion of IL-6, IL-1β, and IL-10 in macrophage cells stimulated with LPS.
Our findings may provide the important information for the precise roles of mmu-miR-27a-5p in the macrophage inflammatory response to LPS stimulation in the future.
American Psychological Association (APA)
Cheng, Ying& Du, Li& Jiao, Hanwei& Zhu, Huapei& Xu, Kailian& Guo, Shiyu…[et al.]. 2015. Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056106
Modern Language Association (MLA)
Cheng, Ying…[et al.]. Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056106
American Medical Association (AMA)
Cheng, Ying& Du, Li& Jiao, Hanwei& Zhu, Huapei& Xu, Kailian& Guo, Shiyu…[et al.]. Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056106
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056106