Expression of the Genes Encoding the Trk and Kdp Potassium Transport Systems of Mycobacterium tuberculosis during Growth In Vitro
Joint Authors
Anderson, Ronald
Cholo, Moloko C.
van Rensburg, Elizabeth J.
Osman, Ayman G.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Two potassium (K+)-uptake systems, Trk and Kdp, are operative in Mycobacterium tuberculosis (Mtb), but the environmental factors triggering their expression have not been determined.
The current study has evaluated the expression of these genes in the Mtb wild-type and a trk-gene knockout strain at various stages of logarithmic growth in relation to extracellular K+ concentrations and pH.
In both strains, mRNA levels of the K+-uptake encoding genes were relatively low compared to those of the housekeeping gene, sigA, at the early- and mid-log phases, increasing during late-log.
Increased gene expression coincided with decreased K+ uptake in the context of a drop in extracellular pH and sustained high extracellular K+ concentrations.
In an additional series of experiments, the pH of the growth medium was manipulated by the addition of 1N HCl/NaOH.
Decreasing the pH resulted in reductions in both membrane potential and K+ uptake in the setting of significant induction of genes encoding both K+ transporters.
These observations are consistent with induction of the genes encoding the active K+ transporters of Mtb as a strategy to compensate for loss of membrane potential-driven uptake of K+ at low extracellular pH.
Induction of these genes may promote survival in the acidic environments of the intracellular vacuole and granuloma.
American Psychological Association (APA)
Cholo, Moloko C.& van Rensburg, Elizabeth J.& Osman, Ayman G.& Anderson, Ronald. 2015. Expression of the Genes Encoding the Trk and Kdp Potassium Transport Systems of Mycobacterium tuberculosis during Growth In Vitro. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056110
Modern Language Association (MLA)
Cholo, Moloko C.…[et al.]. Expression of the Genes Encoding the Trk and Kdp Potassium Transport Systems of Mycobacterium tuberculosis during Growth In Vitro. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056110
American Medical Association (AMA)
Cholo, Moloko C.& van Rensburg, Elizabeth J.& Osman, Ayman G.& Anderson, Ronald. Expression of the Genes Encoding the Trk and Kdp Potassium Transport Systems of Mycobacterium tuberculosis during Growth In Vitro. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056110