Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico
Joint Authors
Barboza-Corona, Jose Eleazar
León-Galván, Ma. Fabiola
Lechuga-Arana, A. Arianna
Valencia-Posadas, Mauricio
Aguayo, Daniel D.
Cedillo-Pelaez, Carlos
Martínez-Ortega, Erika A.
Gutierrez-Chavez, Abner J.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Thirty-two farms (n=535 cows) located in the state of Guanajuato, Mexico, were sampled.
Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins of Bacillus thuringiensis was tested.
Forty-six milk samples were selected for their positive California Mastitis Test (CMT) (≥3) and any abnormality in the udder or milk.
The frequency of SCM and CLM was 39.1% and 9.3%, respectively.
Averages for test day milk yield (MY), lactation number (LN), herd size (HS), and number of days in milk (DM) were 20.6 kg, 2.8 lactations, 16.7 animals, and 164.1 days, respectively.
MY was dependent on dairy herd (DH), LN, HS, and DM P<0.01, and correlations between udder quarters from the CMT were around 0.49 P<0.01.
Coagulase-negative staphylococci were mainly identified, as well as Staphylococcus aureus, Streptococcus uberis, Brevibacterium stationis, B.
conglomeratum, and Staphylococcus agnetis.
Bacterial isolates were resistant to penicillin, clindamycin, ampicillin, and cefotaxime.
Bacteriocins synthesized by Bacillus thuringiensis inhibited the growth of multiantibiotic resistance bacteria such as S.
agnetis, S.
equorum, Streptococcus uberis, Brevibacterium stationis, and Brachybacterium conglomeratum, but they were not active against S.
sciuri, a microorganism that showed an 84% resistance to antibiotics tested in this study.
American Psychological Association (APA)
León-Galván, Ma. Fabiola& Barboza-Corona, Jose Eleazar& Lechuga-Arana, A. Arianna& Valencia-Posadas, Mauricio& Aguayo, Daniel D.& Cedillo-Pelaez, Carlos…[et al.]. 2015. Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056134
Modern Language Association (MLA)
León-Galván, Ma. Fabiola…[et al.]. Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056134
American Medical Association (AMA)
León-Galván, Ma. Fabiola& Barboza-Corona, Jose Eleazar& Lechuga-Arana, A. Arianna& Valencia-Posadas, Mauricio& Aguayo, Daniel D.& Cedillo-Pelaez, Carlos…[et al.]. Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056134
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056134