Monitoring the Modifications of the Vitreous Humor Metabolite Profile after Death: An Animal Model
Joint Authors
Noto, A.
Rosa, Maria Francesca
Scano, Paola
Nioi, Matteo
Sanna, Roberta
Paribello, Francesco
De-Giorgio, Fabio
Locci, Emanuela
d’Aloja, Ernesto
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We applied a metabolomic approach to monitor the modifications occurring in goat vitreous humor (VH) metabolite composition at different times (0, 6, 12, 18, and 24 hours) after death.
The 1H-NMR analysis of the VH samples was performed for the simultaneous determination of several metabolites (i.e., the metabolite profile) representative of the VH status at different times.
Spectral data were analyzed by Principal Component Analysis (PCA) and by Orthogonal Projection to Latent Structures (OPLS) regression technique.
PCA and OPLS suggested that different spectral regions were involved in time-related changes.
The major time-related compositional changes, here detected, were the increase of lactate, hypoxanthine, alanine, total glutathione, choline/phosphocholine, creatine, and myo-inositol and the decrease of glucose and 3-hydroxybutyrate.
We attempted a speculative interpretation of the biological mechanisms underlying these changes.
These results show that multivariate statistical approach, based on 1H NMR metabolite profiling, is a powerful tool for detecting ongoing differences in VH composition and may be applied to investigate several physiological and pathological conditions.
American Psychological Association (APA)
Rosa, Maria Francesca& Scano, Paola& Noto, A.& Nioi, Matteo& Sanna, Roberta& Paribello, Francesco…[et al.]. 2015. Monitoring the Modifications of the Vitreous Humor Metabolite Profile after Death: An Animal Model. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1056189
Modern Language Association (MLA)
Rosa, Maria Francesca…[et al.]. Monitoring the Modifications of the Vitreous Humor Metabolite Profile after Death: An Animal Model. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1056189
American Medical Association (AMA)
Rosa, Maria Francesca& Scano, Paola& Noto, A.& Nioi, Matteo& Sanna, Roberta& Paribello, Francesco…[et al.]. Monitoring the Modifications of the Vitreous Humor Metabolite Profile after Death: An Animal Model. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1056189
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056189