Osteogenic Matrix Cell Sheets Facilitate Osteogenesis in Irradiated Rat Bone
Joint Authors
Morita, Yusuke
Akahane, Manabu
Ueha, Tomoyuki
Shimizu, Takamasa
Kawate, Kenji
Uchihara, Yoshinobu
Nakasaki, Shintaro
Kura, Tomohiko
Tohma, Yasuaki
Kido, Akira
Tanaka, Yasuhito
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Reconstruction of large bone defects after resection of malignant musculoskeletal tumors is a significant challenge in orthopedic surgery.
Extracorporeal autogenous irradiated bone grafting is a treatment option for bone reconstruction.
However, nonunion often occurs because the osteogenic capacity is lost by irradiation.
In the present study, we established an autogenous irradiated bone graft model in the rat femur to assess whether osteogenic matrix cell sheets improve osteogenesis of the irradiated bone.
Osteogenic matrix cell sheets were prepared from bone marrow-derived stromal cells and co-transplanted with irradiated bone.
X-ray images at 4 weeks after transplantation showed bridging callus formation around the irradiated bone.
Micro-computed tomography images at 12 weeks postoperatively showed abundant callus formation in the whole circumference of the irradiated bone.
Histology showed bone union between the irradiated bone and host femur.
Mechanical testing showed that the failure force at the irradiated bone site was significantly higher than in the control group.
Our study indicates that osteogenic matrix cell sheet transplantation might be a powerful method to facilitate osteogenesis in irradiated bones, which may become a treatment option for reconstruction of bone defects after resection of malignant musculoskeletal tumors.
American Psychological Association (APA)
Uchihara, Yoshinobu& Akahane, Manabu& Shimizu, Takamasa& Ueha, Tomoyuki& Morita, Yusuke& Nakasaki, Shintaro…[et al.]. 2015. Osteogenic Matrix Cell Sheets Facilitate Osteogenesis in Irradiated Rat Bone. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1056200
Modern Language Association (MLA)
Uchihara, Yoshinobu…[et al.]. Osteogenic Matrix Cell Sheets Facilitate Osteogenesis in Irradiated Rat Bone. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1056200
American Medical Association (AMA)
Uchihara, Yoshinobu& Akahane, Manabu& Shimizu, Takamasa& Ueha, Tomoyuki& Morita, Yusuke& Nakasaki, Shintaro…[et al.]. Osteogenic Matrix Cell Sheets Facilitate Osteogenesis in Irradiated Rat Bone. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1056200
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056200