Vascular Damage in Resistant Hypertension: TNF-Alpha InhibitionEffects on Endothelial Cells
Joint Authors
Anhe, Gabriel F.
Barbaro, Natalia Ruggeri
Moreno, Heitor
de Araújo, Thiago Matos
Tanus-Santos, José Eduardo
Fontana, Vanessa
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Inflammatory cytokines have been associated with the pathophysiology of hypertension and target organ damage (TOD).
Resistant hypertensive patients (RHTN) are characterized by poor blood pressure control and higher prevalence of TOD.
This study evaluated the relationship between plasma levels of TNF-α and arterial stiffness (pulse wave velocity—PWV) in 32 RHTN and 19 normotensive subjects.
Moreover, we investigated the effect of TNF-α inhibition on human endothelial cells (HUVECs) incubated with serum from RHTN and normotensive subjects.
HUVECs containing serum obtained from normotensive n=8 and hypertensive n=8 individuals were treated with TNF-α inhibitor (infliximab).
Cell suspensions were used for measurement of DNA fragmentation and reactive oxygen species (ROS) content.
RHTN patients showed higher levels of TNF-α compared to normotensive subjects, as well as higher PWV.
Positive correlation was found between TNF-α levels and PWV measures in the whole group.
HUVECs incubated with serum from RHTN showed increased cell apoptosis and higher ROS content compared to normotensive subjects.
Infliximab attenuated the apoptosis of HUVECs incubated with serum from RHTN, but no effect in ROS production was observed.
Our findings suggest that TNF-α might mediate, at least in part, vascular damage in resistant hypertension.
American Psychological Association (APA)
Barbaro, Natalia Ruggeri& de Araújo, Thiago Matos& Tanus-Santos, José Eduardo& Anhe, Gabriel F.& Fontana, Vanessa& Moreno, Heitor. 2015. Vascular Damage in Resistant Hypertension: TNF-Alpha InhibitionEffects on Endothelial Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056212
Modern Language Association (MLA)
Barbaro, Natalia Ruggeri…[et al.]. Vascular Damage in Resistant Hypertension: TNF-Alpha InhibitionEffects on Endothelial Cells. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056212
American Medical Association (AMA)
Barbaro, Natalia Ruggeri& de Araújo, Thiago Matos& Tanus-Santos, José Eduardo& Anhe, Gabriel F.& Fontana, Vanessa& Moreno, Heitor. Vascular Damage in Resistant Hypertension: TNF-Alpha InhibitionEffects on Endothelial Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056212
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056212