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Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover)
Joint Authors
Taha, Rosna Mat
Khorasani Esmaeili, Arash
Banisalam, Behrooz
Mohajer, Sadegh
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
In the present study the extracts of in vivo and in vitro grown plants as well as callus tissue of red clover were tested for their antioxidant activities, using different extraction solvent and different antioxidant assays.
The total flavonoid and phenolic contents as well as extraction yield of the extracts were also investigated to determine their correlation with the antioxidant activity of the extracts.
Among all the tested extracts the highest amounts of total phenolic and total flavonoids content were found in methanol extract of in vivo grown plants.
The antioxidant activity of tested samples followed the order in vivo plant extract > callus extract > in vitro extract.
The highest reducing power, 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, and chelating power were found in methanol extracts of in vivo grown red clover, while the chloroform fraction of in vivo grown plants showed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, superoxide anion radical scavenging and hydrogen peroxide scavenging compared to the other tested extracts.
A significant correlation was found between the antioxidant activity of extracts and their total phenolic and total flavonoid content.
According to the findings, the extract of in vitro culture of red clover especially the callus tissue possesses a comparable antioxidant activity to the in vivo cultured plants’ extract.
American Psychological Association (APA)
Khorasani Esmaeili, Arash& Taha, Rosna Mat& Mohajer, Sadegh& Banisalam, Behrooz. 2015. Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover). BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056257
Modern Language Association (MLA)
Khorasani Esmaeili, Arash…[et al.]. Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover). BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056257
American Medical Association (AMA)
Khorasani Esmaeili, Arash& Taha, Rosna Mat& Mohajer, Sadegh& Banisalam, Behrooz. Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover). BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056257
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056257