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The Osteogenic Properties of Multipotent Mesenchymal Stromal Cells in Cultures on TiO2 Sol-Gel-Derived Biomaterial
Joint Authors
Grzesiak, Jakub
Krzak, Justyna
Śmieszek, Agnieszka
Siudzińska, Anna
Marędziak, Monika
Donesz-Sikorska, Anna
Marycz, Krzysztof
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The biocompatibility of the bone implants is a crucial factor determining the successful tissue regeneration.
The aim of this work was to compare cellular behavior and osteogenic properties of rat adipose-derived multipotent stromal cells (ASCs) and bone marrow multipotent stromal cells (BMSCs) cultured on metallic substrate covered with TiO2 sol-gel-derived nanolayer.
The morphology, proliferation rate, and osteogenic differentiation potential of both ASCs and BMSCs propagated on the biomaterials were examined.
The potential for osteogenic differentiation of ASCs and BMSCs was determined based on the presence of specific markers of osteogenesis, that is, alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCL).
Additionally, the concentration of calcium and phosphorus in extracellular matrix was determined using energy-dispersive X-ray spectroscopy (SEM-EDX).
Obtained results showed that TiO2 layer influenced proliferation activity of ASCs, which manifested by shortening of population doubling time and increase of OPN secretion.
However, characteristic features of cells morphology and growth pattern of cultures prompted us to conclude that ultrathin TiO2 layer might also enhance osteodifferentiation of BMSCs.
Therefore in our opinion, both populations of MSCs should be used for biological evaluation of biomaterials compatibility, such results may enhance the area of investigations related to regenerative medicine.
American Psychological Association (APA)
Marycz, Krzysztof& Śmieszek, Agnieszka& Grzesiak, Jakub& Siudzińska, Anna& Marędziak, Monika& Donesz-Sikorska, Anna…[et al.]. 2015. The Osteogenic Properties of Multipotent Mesenchymal Stromal Cells in Cultures on TiO2 Sol-Gel-Derived Biomaterial. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056290
Modern Language Association (MLA)
Marycz, Krzysztof…[et al.]. The Osteogenic Properties of Multipotent Mesenchymal Stromal Cells in Cultures on TiO2 Sol-Gel-Derived Biomaterial. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056290
American Medical Association (AMA)
Marycz, Krzysztof& Śmieszek, Agnieszka& Grzesiak, Jakub& Siudzińska, Anna& Marędziak, Monika& Donesz-Sikorska, Anna…[et al.]. The Osteogenic Properties of Multipotent Mesenchymal Stromal Cells in Cultures on TiO2 Sol-Gel-Derived Biomaterial. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056290
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056290