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Discrepancies in Drug Susceptibility Test for Tuberculosis Patients Resulted from the Mixed Infection and the Testing System
Joint Authors
Mei, Zaoxian
Sun, Zhaogang
Bai, Dapeng
Xu, Yuhui
Li, Zhiling
Huang, Hairong
Li, Chuanyou
Xu, Shaofa
Li, Li
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
To find the potential reasons for the discrepancies in the drug susceptibility test (DST) of M.
tuberculosis isolates, twenty paired isolates with disputed drug susceptibilities to isoniazid (INH) were selected according to the MGIT960 testing and Löwenstein-Jensen (L-J) proportion methods.
Their MICs were confirmed again by broth microdilution method and by L-J proportion method.
The spoligotyping results showed that, of all the 20 paired strains, 11 paired isolates belonged to the Beijing genotype and 6 paired isolates belonged to SIT1634, and that each of the remaining 3 paired isolates had two genotypes, namely, SIT1 and SIT1634.
Those 3 paired isolates with different intrapair spoligotypes were further confirmed as mixed infection by the results that those three pairs of isolates with different 12 locus MIRU intrapair types and one pair carried different base pair at codon 315 (AGC versus AAC).
Totally mutations in the katG gene were identified in 13 paired isolates.
No mutations were found in the regulatory sequences and open reading frames (ORF) of the inhA and ahpC genes in any of the tested isolates.
Those results showed that the different test systems and the mixed infection with particular genotypes of M.
tuberculosis strains contributed to the drug susceptibility discrepancies.
American Psychological Association (APA)
Mei, Zaoxian& Sun, Zhaogang& Bai, Dapeng& Xu, Yuhui& Li, Zhiling& Huang, Hairong…[et al.]. 2015. Discrepancies in Drug Susceptibility Test for Tuberculosis Patients Resulted from the Mixed Infection and the Testing System. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1056296
Modern Language Association (MLA)
Mei, Zaoxian…[et al.]. Discrepancies in Drug Susceptibility Test for Tuberculosis Patients Resulted from the Mixed Infection and the Testing System. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1056296
American Medical Association (AMA)
Mei, Zaoxian& Sun, Zhaogang& Bai, Dapeng& Xu, Yuhui& Li, Zhiling& Huang, Hairong…[et al.]. Discrepancies in Drug Susceptibility Test for Tuberculosis Patients Resulted from the Mixed Infection and the Testing System. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1056296
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056296