Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells
Joint Authors
Zambrano, Nicola
Sasso, Emanuele
Monteleone, Francesca
Boffo, Francesca Ludovica
Santoriello, Margherita
Sarnataro, Daniela
Garbi, Corrado
Sabatella, Mariangela
Crifò, Bianca
Paolella, Luca Alfredo
Minopoli, Giuseppina
Winum, Jean-Yves
Vitale, Monica
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Carbonic anhydrase IX (CA IX) is a surrogate marker of hypoxia, involved in survival and pH regulation in hypoxic cells.
We have recently characterized its interactome, describing a set of proteins interacting with CA IX, mainly in hypoxic cells, including several members of the nucleocytoplasmic shuttling apparatuses.
Accordingly, we described complex subcellular localization for this enzyme in human cells, as well as the redistribution of a carbonic anhydrase IX pool to nucleoli during hypoxia.
Starting from this evidence, we analyzed the possible contribution of carbonic anhydrase IX to transcription of the 45S rDNA genes, a process occurring in nucleoli.
We highlighted the binding of carbonic anhydrase IX to nucleolar chromatin, which is regulated by oxygen levels.
In fact, CA IX was found on 45S rDNA gene promoters in normoxic cells and less represented on these sites, in hypoxic cells and in cells subjected to acetazolamide-induced acidosis.
Both conditions were associated with increased representation of carbonic anhydrase IX/exportin-1 complexes in nucleoli.
45S rRNA transcript levels were accordingly downrepresented.
Inhibition of nuclear export by leptomycin B suggests a model in which exportin-1 acts as a decoy, in hypoxic cells, preventing carbonic anhydrase IX association with 45S rDNA gene promoters.
American Psychological Association (APA)
Sasso, Emanuele& Vitale, Monica& Monteleone, Francesca& Boffo, Francesca Ludovica& Santoriello, Margherita& Sarnataro, Daniela…[et al.]. 2015. Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056346
Modern Language Association (MLA)
Sasso, Emanuele…[et al.]. Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056346
American Medical Association (AMA)
Sasso, Emanuele& Vitale, Monica& Monteleone, Francesca& Boffo, Francesca Ludovica& Santoriello, Margherita& Sarnataro, Daniela…[et al.]. Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056346
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056346