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Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs
Joint Authors
Ji, Shengyue
Li, Weili
Xin, Haiyun
Wang, Shan
Cao, Binyun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Sublancin 168, as a distinct S-linked antimicrobial glycopeptide produced by Bacillus subtilis 168, is effective in killing specific microorganisms.
However, the reported yield of sublancin 168 is at a low level of no more than 60 mg from 1 L fermentation culture of B.
subtilis 168 by using the method in the literature.
Thus optimization of fermentation condition for efficiently producing sublancin 168 is required.
Here, Box-Behnken design was used to determine the optimal combination of three fermentation parameters, namely, corn powder, soybean meal, and temperature that were identified previously by Plackett-Burman design and the steepest ascent experiment.
Subsequently, based on the response surface methodology, the quadratic regression model for optimally producing sublancin 168 was developed, and the optimal combination of culture parameters for maximum sublancin 168 production of 129.72 mg/L was determined as corn powder 28.49 g/L, soybean meal 22.99 g/L, and incubation temperature 30.8°C.
The results showed that sublancin 168 production obtained experimentally was coincident with predicted value of 125.88 mg/L, and the developed model was proved to be adequate, and the aim of efficiently producing sublancin 168 was achieved.
American Psychological Association (APA)
Ji, Shengyue& Li, Weili& Xin, Haiyun& Wang, Shan& Cao, Binyun. 2015. Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056394
Modern Language Association (MLA)
Ji, Shengyue…[et al.]. Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056394
American Medical Association (AMA)
Ji, Shengyue& Li, Weili& Xin, Haiyun& Wang, Shan& Cao, Binyun. Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056394
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056394