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Rotavirus Infects Human Biliary Epithelial Cells and Stimulates Secretion of Cytokines IL-6 and IL-8 via MAPK Pathway
Joint Authors
Clemente, Maria Grazia
Patton, John T.
Anders, Robert A.
Yolken, Robert H.
Schwarz, Kathleen B.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Biliary atresia (BA) is an infantile inflammatory cholangiopathy of unknown etiology although epidemiologic studies and animal models utilizing rotavirus (RV) have suggested a role for viral infection.
Proinflammatory and profibrotic cytokines have been detected in infants with BA.
The purpose of our study was to investigate the susceptibility of human cholangiocytes (H69 cells) to infection with RRV and to determine if this infection resulted in cytokine secretion.
Infection of H69 cells by RRV was noncytolytic and resulted in a time-dependent increase in the release of both infectious virions and cytokines IL-6 and IL-8 into the supernate.
The greatest difference in cytokine supernatant levels between infected and mock-infected cells was noted at 24 hours postinfection (h p.i.) for IL-8, 556 ± 111 versus 77 ± 68 pg/mL ( p < 0.0001 ), and at 48 h p.i.
for IL-6, 459 ± 64 versus 67 ± 2 pg/mL ( p < 0.0001 ).
Production of both cytokines following RRV infection was significantly reduced by pretreating the H69 cells with inhibitors of mitogen-activated protein kinase (MAPK).
Conclusion.
RRV can infect human cholangiocytes resulting in the production of proinflammatory and profibrotic cytokines via the MAPK pathway.
RRV-infected H69 cells could be a useful model system for investigating the viral hypothesis of BA.
American Psychological Association (APA)
Clemente, Maria Grazia& Patton, John T.& Anders, Robert A.& Yolken, Robert H.& Schwarz, Kathleen B.. 2015. Rotavirus Infects Human Biliary Epithelial Cells and Stimulates Secretion of Cytokines IL-6 and IL-8 via MAPK Pathway. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056420
Modern Language Association (MLA)
Clemente, Maria Grazia…[et al.]. Rotavirus Infects Human Biliary Epithelial Cells and Stimulates Secretion of Cytokines IL-6 and IL-8 via MAPK Pathway. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056420
American Medical Association (AMA)
Clemente, Maria Grazia& Patton, John T.& Anders, Robert A.& Yolken, Robert H.& Schwarz, Kathleen B.. Rotavirus Infects Human Biliary Epithelial Cells and Stimulates Secretion of Cytokines IL-6 and IL-8 via MAPK Pathway. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056420