The J-Domain of Heat Shock Protein 40 Can Enhance the Transduction Efficiency of Arginine-Rich Cell-Penetrating Peptides
Joint Authors
Lin, Tzu-Yin
Su, Yu-Hsiu
Lee, Kun-Hsiung
Chuang, Chin-Kai
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sense and antisense oligonucleotide pairs encoding cell-penetrating peptides PTD (Tat47–57), DPV3A, E162, pVEC, R11, and TP13 were used to construct two sets of pET22b-CPP-DsRed and pET22b-CPP-J-DsRed vectors for CPP-DsRed and CPP-J-DsRed recombinant proteins expression.
PTD-DsRed, DPV3A-DsRed, PTD-J-DsRed, and DPV3A-J-DsRed recombinant proteins were expressed in a soluble form.
PTD-J-DsRed and DPV3A-J-DsRed recombinant proteins were able to escape from E.
coli host cells into the culture medium.
The membrane-penetrating activity of PTD-J-DsRed and DPV3A-J-DsRed recombinant proteins to mammalian cells was more effective than that of PTD-DsRed and DPV3A-DsRed.
The route of the cellular membrane translocation of these recombinant proteins is suggested via macropinocytosis followed by an endosomal escape pathway.
American Psychological Association (APA)
Lin, Tzu-Yin& Su, Yu-Hsiu& Lee, Kun-Hsiung& Chuang, Chin-Kai. 2015. The J-Domain of Heat Shock Protein 40 Can Enhance the Transduction Efficiency of Arginine-Rich Cell-Penetrating Peptides. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056424
Modern Language Association (MLA)
Lin, Tzu-Yin…[et al.]. The J-Domain of Heat Shock Protein 40 Can Enhance the Transduction Efficiency of Arginine-Rich Cell-Penetrating Peptides. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056424
American Medical Association (AMA)
Lin, Tzu-Yin& Su, Yu-Hsiu& Lee, Kun-Hsiung& Chuang, Chin-Kai. The J-Domain of Heat Shock Protein 40 Can Enhance the Transduction Efficiency of Arginine-Rich Cell-Penetrating Peptides. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056424
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056424