One-Step Recovery of scFv Clones from High-Throughput Sequencing-Based Screening of Phage Display Libraries Challenged to Cells Expressing Native Claudin-1
Joint Authors
Zambrano, Nicola
Sasso, Emanuele
Paciello, Rolando
D’Auria, Francesco
Riccio, Gennaro
Froechlich, Guendalina
Cortese, Riccardo
Nicosia, Alfredo
De Lorenzo, Claudia
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Expanding the availability of monoclonal antibodies interfering with hepatitis C virus infection of hepatocytes is an active field of investigation within medical biotechnologies, to prevent graft reinfection in patients subjected to liver transplantation and to overcome resistances elicited by novel antiviral drugs.
In this paper, we describe a complete pipeline for screening of phage display libraries of human scFvs against native Claudin-1, a tight-junction protein involved in hepatitis C virus infection, expressed on the cell surface of human hepatocytes.
To this aim, we implemented a high-throughput sequencing approach for library screening, followed by a simple and effective strategy to recover active binder clones from enriched sublibraries.
The recovered clones were successfully converted to active immunoglobulins, thus demonstrating the effectiveness of the whole procedure.
This novel approach can guarantee rapid and cheap isolation of antibodies for virtually any native antigen involved in human diseases, for therapeutic and/or diagnostic applications.
American Psychological Association (APA)
Sasso, Emanuele& Paciello, Rolando& D’Auria, Francesco& Riccio, Gennaro& Froechlich, Guendalina& Cortese, Riccardo…[et al.]. 2015. One-Step Recovery of scFv Clones from High-Throughput Sequencing-Based Screening of Phage Display Libraries Challenged to Cells Expressing Native Claudin-1. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056431
Modern Language Association (MLA)
Sasso, Emanuele…[et al.]. One-Step Recovery of scFv Clones from High-Throughput Sequencing-Based Screening of Phage Display Libraries Challenged to Cells Expressing Native Claudin-1. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056431
American Medical Association (AMA)
Sasso, Emanuele& Paciello, Rolando& D’Auria, Francesco& Riccio, Gennaro& Froechlich, Guendalina& Cortese, Riccardo…[et al.]. One-Step Recovery of scFv Clones from High-Throughput Sequencing-Based Screening of Phage Display Libraries Challenged to Cells Expressing Native Claudin-1. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056431
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056431