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Ascorbic Acid, Ultraviolet C Rays, and Glucose but not Hyperthermia Are Elicitors of Human β-Defensin 1 mRNA in Normal Keratinocytes
Joint Authors
Cruz Díaz, Luis Antonio
Flores Miramontes, María Guadalupe
Chávez Hurtado, Paulina
Allen, Kirk
Gonzalez Ávila, Marisela
Prado Montes de Oca, Ernesto
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Hosts’ innate defense systems are upregulated by antimicrobial peptide elicitors (APEs).
Our aim was to investigate the effects of hyperthermia, ultraviolet A rays (UVA), and ultraviolet C rays (UVC) as well as glucose and ascorbic acid (AA) on the regulation of human β-defensin 1 (DEFB1), cathelicidin (CAMP), and interferon-γ (IFNG) genes in normal human keratinocytes (NHK).
The indirect in vitro antimicrobial activity against Staphylococcus aureus and Listeria monocytogenes of these potential APEs was tested.
We found that AA is a more potent APE for DEFB1 than glucose in NHK.
Glucose but not AA is an APE for CAMP.
Mild hypo- (35°C) and hyperthermia (39°C) are not APEs in NHK.
AA-dependent DEFB1 upregulation below 20 mM predicts in vitro antimicrobial activity as well as glucose- and AA-dependent CAMP and IFNG upregulation.
UVC upregulates CAMP and DEFB1 genes but UVA only upregulates the DEFB1 gene.
UVC is a previously unrecognized APE in human cells.
Our results suggest that glucose upregulates CAMP in an IFN-γ-independent manner.
AA is an elicitor of innate immunity that will challenge the current concept of late activation of adaptive immunity of this vitamin.
These results could be useful in designing new potential drugs and devices to combat skin infections.
American Psychological Association (APA)
Cruz Díaz, Luis Antonio& Flores Miramontes, María Guadalupe& Chávez Hurtado, Paulina& Allen, Kirk& Gonzalez Ávila, Marisela& Prado Montes de Oca, Ernesto. 2015. Ascorbic Acid, Ultraviolet C Rays, and Glucose but not Hyperthermia Are Elicitors of Human β-Defensin 1 mRNA in Normal Keratinocytes. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056474
Modern Language Association (MLA)
Cruz Díaz, Luis Antonio…[et al.]. Ascorbic Acid, Ultraviolet C Rays, and Glucose but not Hyperthermia Are Elicitors of Human β-Defensin 1 mRNA in Normal Keratinocytes. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056474
American Medical Association (AMA)
Cruz Díaz, Luis Antonio& Flores Miramontes, María Guadalupe& Chávez Hurtado, Paulina& Allen, Kirk& Gonzalez Ávila, Marisela& Prado Montes de Oca, Ernesto. Ascorbic Acid, Ultraviolet C Rays, and Glucose but not Hyperthermia Are Elicitors of Human β-Defensin 1 mRNA in Normal Keratinocytes. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056474