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A Photoperiod-Regulating Gene CONSTANS Is Correlated to Lipid Biosynthesis in Chlamydomonas reinhardtii
Joint Authors
Fei, Xiaowen
Fan, Xinzhao
Li, Ping
Deng, Xiaodong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
The regulation of lipid biosynthesis is essential in photosynthetic eukaryotic cells.
Thus far, no regulatory genes have been reported in the lipid metabolism pathway.
Plant CONSTANS (CO) gene regulates blooming by participating in photoperiod and biological clock.
Apart from regulating photoperiod, the Chlamydomonas CO gene also regulates starch content.
Results.
In this study, the results showed that, under HSM-S condition, cells accumulated more lipids at short-day conditions than at long-day conditions.
The silencing of the CrCO gene via RNA interference resulted in an increase in lipid content and an increase in triacylglyceride (TAG) level by 24.5%.
CrCO RNAi strains accumulated more lipids at short-day conditions than at long-day conditions.
The decrease in CrCO expression resulted in the increased expression of TAG biosynthesis-related genes, such as DGAT2, PAP2, and PDAT3, whereas CIS and FBP1 genes showed a decrease in their mRNA when the CrCO expression was suppressed.
On the other hand, the overexpression of CrCO resulted in the decrease in lipid content and TAG level.
Conclusions.
The results of this study revealed a relationship between CrCO gene and lipid metabolism in Chlamydomonas, suggesting that increasing oil by suppressing CrCO expression in microalgae is feasible.
American Psychological Association (APA)
Deng, Xiaodong& Fan, Xinzhao& Li, Ping& Fei, Xiaowen. 2015. A Photoperiod-Regulating Gene CONSTANS Is Correlated to Lipid Biosynthesis in Chlamydomonas reinhardtii. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056476
Modern Language Association (MLA)
Deng, Xiaodong…[et al.]. A Photoperiod-Regulating Gene CONSTANS Is Correlated to Lipid Biosynthesis in Chlamydomonas reinhardtii. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1056476
American Medical Association (AMA)
Deng, Xiaodong& Fan, Xinzhao& Li, Ping& Fei, Xiaowen. A Photoperiod-Regulating Gene CONSTANS Is Correlated to Lipid Biosynthesis in Chlamydomonas reinhardtii. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056476
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056476