Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Trichosporon asahii in Experimental and Clinical Samples
Joint Authors
Zhou, Jianfeng
Liao, Yong
Li, Haitao
Lu, Xuelian
Han, Xiufeng
Tian, Yanli
Chen, Shanshan
Yang, Rongya
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Invasive trichosporonosis is a deep mycosis found mainly in immunocompromised hosts, and the major pathogen is Trichosporon asahii.
We detected the species-specific intergenic spacers (IGS) of rRNA gene of T.
asahii using a loop-mediated isothermal amplification (LAMP) assay in 15 isolates with 3 different visualization methods, including SYBR green detection, gel electrophoresis, and turbidimetric methods.
The LAMP assay displayed superior rapidity to other traditional methods in the detection time; that is, only 1 h was needed for detection and identification of the pathogen DNA.
Furthermore, the detection limit of the LAMP assay was more sensitive than the PCR assay.
We also successfully detect the presence of T.
asahii in samples from experimentally infected mice and samples from patients with invasive trichosporonosis caused by T.
asahii, suggesting that this method may become useful in clinical applications in the near future.
American Psychological Association (APA)
Zhou, Jianfeng& Liao, Yong& Li, Haitao& Lu, Xuelian& Han, Xiufeng& Tian, Yanli…[et al.]. 2015. Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Trichosporon asahii in Experimental and Clinical Samples. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056557
Modern Language Association (MLA)
Zhou, Jianfeng…[et al.]. Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Trichosporon asahii in Experimental and Clinical Samples. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056557
American Medical Association (AMA)
Zhou, Jianfeng& Liao, Yong& Li, Haitao& Lu, Xuelian& Han, Xiufeng& Tian, Yanli…[et al.]. Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Trichosporon asahii in Experimental and Clinical Samples. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056557
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056557