Nordihydroguaiaretic Acid Disrupts the Antioxidant Ability of Helicobacter pylori through the Repression of SodB Activity In Vitro
Joint Authors
Suzuki, Hidekazu
Tsugawa, Hitoshi
Mori, Hideki
Matsuzaki, Juntaro
Masaoka, Tatsuhiro
Hirayama, Tasuku
Nagasawa, Hideko
Sakakibara, Yasubumi
Suematsu, Makoto
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Iron-cofactored superoxide dismutase (SodB) of Helicobacter pylori plays an indispensable role in the bacterium’s colonization of the stomach.
Previously, we demonstrated that FecA1, a Fe3+-dicitrate transporter homolog, contributes to SodB activation by supplying ferrous iron (Fe2+) to SodB, and fecA1-deletion mutant strains have reduced gastric mucosal-colonization ability in Mongolian gerbils, suggesting that FecA1 is a possible target for the development of a novel eradication therapy.
This study aimed to identify novel FecA1-binding compounds in silico and then examined the effect of a predicted FecA1-binding compound on H.
pylori SodB activity in vitro.
Specifically, we demonstrated that nordihydroguaiaretic acid (NDGA) is a predicted FecA1-binding compound.
NDGA reduced intracellular Fe2+ levels in H.
pylori and reduced SodB activity.
Additionally, NDGA increased H2O2 sensitivity of H.
pylori and increased the metronidazole (Mtz) sensitivity.
The present study demonstrated that NDGA repressed SodB activity associated with the gastric mucosal-colonization via inhibition of intracellular Fe2+ uptake by FecA1, suggesting that NDGA might be effective for the development of a novel eradication therapy.
American Psychological Association (APA)
Tsugawa, Hitoshi& Mori, Hideki& Matsuzaki, Juntaro& Masaoka, Tatsuhiro& Hirayama, Tasuku& Nagasawa, Hideko…[et al.]. 2015. Nordihydroguaiaretic Acid Disrupts the Antioxidant Ability of Helicobacter pylori through the Repression of SodB Activity In Vitro. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056561
Modern Language Association (MLA)
Tsugawa, Hitoshi…[et al.]. Nordihydroguaiaretic Acid Disrupts the Antioxidant Ability of Helicobacter pylori through the Repression of SodB Activity In Vitro. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056561
American Medical Association (AMA)
Tsugawa, Hitoshi& Mori, Hideki& Matsuzaki, Juntaro& Masaoka, Tatsuhiro& Hirayama, Tasuku& Nagasawa, Hideko…[et al.]. Nordihydroguaiaretic Acid Disrupts the Antioxidant Ability of Helicobacter pylori through the Repression of SodB Activity In Vitro. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056561
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056561