Generation of Neurospheres from Human Adipose-Derived Stem Cells
Joint Authors
Yang, Erfang
Liu, Na
Tang, Yingxin
Hu, Yang
Zhang, Ping
Pan, Chao
Dong, Shasha
Zhang, Youping
Tang, Zhouping
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Transplantation of neural stem cells (NSCs) to treat neurodegenerative disease shows promise; however, the clinical application of NSCs is limited by the invasive procurement and ethical concerns.
Adipose-derived stem cells (ADSCs) are a source of multipotent stem cells that can self-renew and differentiate into various kinds of cells; this study intends to generate neurospheres from human ADSCs by culturing ADSCs on uncoated culture flasks in serum-free neurobasal medium supplemented with B27, basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF); the ADSCs-derived neurospheres were terminally differentiated after growth factor withdrawal.
Expression of Nestin, NeuN, MAP2, and GFAP in ADSCs and terminally differentiated neurospheres was shown by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blotting, and immunocytochemistry; cell proliferation in neurospheres was evaluated by cell cycle analyses, immunostaining, and flow cytometry.
These data strongly support the conclusion that human ADSCs can successfully differentiate into neurospheres efficiently on uncoated culture flasks, which present similar molecular marker pattern and proliferative ability with NSCs derived from embryonic and adult brain tissues.
Therefore, human ADSCs may be an ideal alternative source of stem cells for the treatment of neurodegenerative diseases.
American Psychological Association (APA)
Yang, Erfang& Liu, Na& Tang, Yingxin& Hu, Yang& Zhang, Ping& Pan, Chao…[et al.]. 2015. Generation of Neurospheres from Human Adipose-Derived Stem Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056589
Modern Language Association (MLA)
Yang, Erfang…[et al.]. Generation of Neurospheres from Human Adipose-Derived Stem Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056589
American Medical Association (AMA)
Yang, Erfang& Liu, Na& Tang, Yingxin& Hu, Yang& Zhang, Ping& Pan, Chao…[et al.]. Generation of Neurospheres from Human Adipose-Derived Stem Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056589
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056589