![](/images/graphics-bg.png)
Isolation and Characterization of Human Lung Lymphatic Endothelial Cells
Joint Authors
Graiani, Gallia
Lorusso, Bruno
Falco, Angela
Madeddu, Denise
Frati, Caterina
Cavalli, Stefano
Gervasi, Andrea
Rinaldi, Laura
Lagrasta, Costanza
Maselli, Davide
Gnetti, Letizia
Silini, Enrico M.
Quaini, Eugenio
Ampollini, Luca
Carbognani, Paolo
Quaini, F.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung.
We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs).
Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied.
A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs.
The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays.
Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation.
HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.
American Psychological Association (APA)
Lorusso, Bruno& Falco, Angela& Madeddu, Denise& Frati, Caterina& Cavalli, Stefano& Graiani, Gallia…[et al.]. 2015. Isolation and Characterization of Human Lung Lymphatic Endothelial Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056606
Modern Language Association (MLA)
Lorusso, Bruno…[et al.]. Isolation and Characterization of Human Lung Lymphatic Endothelial Cells. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1056606
American Medical Association (AMA)
Lorusso, Bruno& Falco, Angela& Madeddu, Denise& Frati, Caterina& Cavalli, Stefano& Graiani, Gallia…[et al.]. Isolation and Characterization of Human Lung Lymphatic Endothelial Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056606
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056606