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Hypergravity Stimulation Enhances PC12 Neuron-Like Cell Differentiation
Joint Authors
Monici, Monica
Mazzolai, Barbara
Mattoli, Virgilio
Cialdai, Francesca
Genchi, Giada Graziana
Ciofani, Gianni
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Altered gravity is a strong physical cue able to elicit different cellular responses, representing a largely uninvestigated opportunity for tissue engineering/regenerative medicine applications.
Our recent studies have shown that both proliferation and differentiation of C2C12 skeletal muscle cells can be enhanced by hypergravity treatment; given these results, PC12 neuron-like cells were chosen to test the hypothesis that hypergravity stimulation might also affect the behavior of neuronal cells, in particular promoting an enhanced differentiated phenotype.
PC12 cells were thus cultured under differentiating conditions for either 12 h or 72 h before being stimulated with different values of hypergravity (50 g and 150 g).
Effects of hypergravity were evaluated at transcriptional level 1 h and 48 h after the stimulation, and at protein level 48 h from hypergravity exposure, to assess its influence on neurite development over increasing differentiation times.
PC12 differentiation resulted strongly affected by the hypergravity treatments; in particular, neurite length was significantly enhanced after exposure to high acceleration values.
The achieved results suggest that hypergravity might induce a faster and higher neuronal differentiation and encourage further investigations on the potential of hypergravity in the preparation of cellular constructs for regenerative medicine and tissue engineering purposes.
American Psychological Association (APA)
Genchi, Giada Graziana& Cialdai, Francesca& Monici, Monica& Mazzolai, Barbara& Mattoli, Virgilio& Ciofani, Gianni. 2015. Hypergravity Stimulation Enhances PC12 Neuron-Like Cell Differentiation. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056607
Modern Language Association (MLA)
Genchi, Giada Graziana…[et al.]. Hypergravity Stimulation Enhances PC12 Neuron-Like Cell Differentiation. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056607
American Medical Association (AMA)
Genchi, Giada Graziana& Cialdai, Francesca& Monici, Monica& Mazzolai, Barbara& Mattoli, Virgilio& Ciofani, Gianni. Hypergravity Stimulation Enhances PC12 Neuron-Like Cell Differentiation. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056607
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056607