Prevalence of Helicobacter pylori vacA, cagA, and iceA Genotypes in Cuban Patients with Upper Gastrointestinal Diseases
Joint Authors
Feliciano, Onelkis
Gutierrez, Oderay
Valdés, Lidunka
Fragoso, Trini
Calderin, Ana Maria
Valdes, Antonio Eduardo
Llanes, Rafael
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-06
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Virulence factors of Helicobacter pylori can predict the development of different gastroduodenal diseases.
There are scarce reports in Cuba about H.
pylori isolates genotyping.
The aim of the present investigation was to identify allelic variation of the virulence genes vacA, cagA, and iceA in sixty-eight patients diagnosed as H.
pylori positive by culture.
In seven out of 68 patients, strains from both gastric regions were obtained and considered independent.
DNA was extracted from all the H.
pylori strains and evaluated by PCR-genotyping.
The vacA s1 allele, cagA gene, and iceA2 allele were the most prevalent (72.0%, 56.0%, and 57.3%, respectively).
Alleles from m-region showed a similar frequency as s1a and s1b subtypes.
The presence of multiple H.
pylori genotypes in a single biopsy and two gastric region specimens were found.
Significant statistical association was observed between iceA2 allele and patients with non-peptic ulcer dyspepsia (NUD) (P=0.037) as well as virulence genotypes (s1, s1m2) and patients over 40 years old (P<0.05).
In conclusion, the results demonstrated a high prevalence of H.
pylori virulent genotypes in Cuban patients over 40 years old while iceA2 alleles demonstrated a good specificity in patients with NUD.
American Psychological Association (APA)
Feliciano, Onelkis& Gutierrez, Oderay& Valdés, Lidunka& Fragoso, Trini& Calderin, Ana Maria& Valdes, Antonio Eduardo…[et al.]. 2015. Prevalence of Helicobacter pylori vacA, cagA, and iceA Genotypes in Cuban Patients with Upper Gastrointestinal Diseases. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1056633
Modern Language Association (MLA)
Feliciano, Onelkis…[et al.]. Prevalence of Helicobacter pylori vacA, cagA, and iceA Genotypes in Cuban Patients with Upper Gastrointestinal Diseases. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1056633
American Medical Association (AMA)
Feliciano, Onelkis& Gutierrez, Oderay& Valdés, Lidunka& Fragoso, Trini& Calderin, Ana Maria& Valdes, Antonio Eduardo…[et al.]. Prevalence of Helicobacter pylori vacA, cagA, and iceA Genotypes in Cuban Patients with Upper Gastrointestinal Diseases. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1056633
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056633