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RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures
Joint Authors
Morabito, Caterina
Steimberg, Nathalie
Mazzoleni, Giovanna
Guarnieri, Simone
Fanò-Illic, Giorgio
Mariggiò, Maria A.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-13
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
We propose a human-derived neuro-/glial cell three-dimensional in vitro model to investigate the effects of microgravity on cell-cell interactions.
A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL15 and neuronal-like SH-SY5Y cells in three-dimensional individual cultures (monotypic aggregates) and cocultures (heterotypic aggregates) were analysed.
Cell survival was maintained within all cell aggregates over 2 weeks of culture.
Moreover, compared to cells as traditional static monolayers, cell aggregates cultured under modelled microgravity showed increased expression of specific differentiation markers (e.g., GL15 cells: GFAP, S100B; SH-SY5Y cells: GAP43) and modulation of functional cell-cell interactions (e.g., N-CAM and Cx43 expression and localisation).
In conclusion, this culture model opens a wide range of specific investigations at the molecular, biochemical, and morphological levels, and it represents an important tool for in vitro studies into dynamic interactions and responses of nervous system cell components to microgravity environmental conditions.
American Psychological Association (APA)
Morabito, Caterina& Steimberg, Nathalie& Mazzoleni, Giovanna& Guarnieri, Simone& Fanò-Illic, Giorgio& Mariggiò, Maria A.. 2015. RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures. BioMed Research International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1056634
Modern Language Association (MLA)
Morabito, Caterina…[et al.]. RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures. BioMed Research International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1056634
American Medical Association (AMA)
Morabito, Caterina& Steimberg, Nathalie& Mazzoleni, Giovanna& Guarnieri, Simone& Fanò-Illic, Giorgio& Mariggiò, Maria A.. RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1056634
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056634