Extracellular Calcium-Dependent Modulation of Endothelium Relaxation in Rat Mesenteric Small Artery: The Role of Potassium Signaling
Joint Authors
Hangaard, Lise
Jessen, Peter B.
Kamaev, Dmitrii
Aalkjaer, Christian
Matchkov, Vladimir V.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The nature of NO- and COX-independent endothelial hyperpolarization (EDH) is not fully understood but activation of small- and intermittent-conductance Ca2+-activated K+ channels ( SK Ca and IK Ca ) is important.
Previous studies have suggested that the significance of IK Ca depends on Ca 2 + out .
Also it has been suggested that K+ is important through localized K + out signaling causing activation of the Na+,K+-ATPase and inward-rectifying K+ channels ( K ir ).
Here we tested the hypothesis that the modulating effect of Ca 2 + out on the EDH-like response depends on K + out .
We addressed this possibility using isometric myography of rat mesenteric small arteries.
When K + out was 4.2 mM, relaxation to acetylcholine (ACh) was stronger at 2.5 mM Ca 2 + out than at 1 mM Ca 2 + out .
Inhibition of IK Ca with TRAM34 suppressed the relaxations but did not change the relation between the relaxations at the low and high Ca 2 + out .
This Ca 2 + out -dependence disappeared at 5.9 mM K + out and in the presence of ouabain or BaCl2.
Our results suggest that IK Ca are involved in the localized K + out signaling which acts through the Na+,K+-ATPase and K ir channels and that the significance of this endothelium-dependent pathway is modulated by Ca 2 + out .
American Psychological Association (APA)
Hangaard, Lise& Jessen, Peter B.& Kamaev, Dmitrii& Aalkjaer, Christian& Matchkov, Vladimir V.. 2015. Extracellular Calcium-Dependent Modulation of Endothelium Relaxation in Rat Mesenteric Small Artery: The Role of Potassium Signaling. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056647
Modern Language Association (MLA)
Hangaard, Lise…[et al.]. Extracellular Calcium-Dependent Modulation of Endothelium Relaxation in Rat Mesenteric Small Artery: The Role of Potassium Signaling. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056647
American Medical Association (AMA)
Hangaard, Lise& Jessen, Peter B.& Kamaev, Dmitrii& Aalkjaer, Christian& Matchkov, Vladimir V.. Extracellular Calcium-Dependent Modulation of Endothelium Relaxation in Rat Mesenteric Small Artery: The Role of Potassium Signaling. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056647
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056647