Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice
Joint Authors
Yan, Fei
Yang, Wei
Li, Xiang
Liu, Hongmei
Nan, Xiang
Xie, Lisi
Zhou, Dongliang
Xie, Guoxi
Wu, Junru
Qiu, Bensheng
Liu, Xin
Zheng, Hairong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The goal of this study is to investigate the feasibility of using CD81- (Cluster of Differentiation 81 protein-) targeted microparticles of iron oxide (CD81-MPIO) for magnetic resonance imaging (MRI) of the murine atherosclerosis.
CD81-MPIO and IgG- (Immunoglobulin G-) MPIO were prepared by covalently conjugating, respectively, with anti-CD81 monoclonal and IgG antibodies to the surface of the tosyl activated MPIO.
The relevant binding capability of the MPIO was examined by incubating them with murine bEnd.3 cells stimulated with phenazine methosulfate (PMS) and its effect in shortening T2 relaxation time was also examined.
MRI in apolipoprotein E-deficient mice was studied in vivo.
Our results show that CD81-MPIO, but not IgG-MPIO, can bind to the PMS-stimulated bEnd.3 cells.
The T2 relaxation time was significantly shortened for stimulated bEnd.3 cells when compared with IgG-MPIO.
In vivo MRI in apolipoprotein E-deficient mice showed highly conspicuous areas of low signal after CD81-MPIO injection.
Quantitative analysis of the area of CD81-MPIO contrast effects showed 8.96- and 6.98-fold increase in comparison with IgG-MPIO or plain MPIO, respectively (P<0.01).
Histological assay confirmed the expression of CD81 and CD81-MPIO binding onto atherosclerotic lesions.
In conclusion, CD81-MPIO allows molecular assessment of murine atherosclerotic lesions by magnetic resonance imaging.
American Psychological Association (APA)
Yan, Fei& Yang, Wei& Li, Xiang& Liu, Hongmei& Nan, Xiang& Xie, Lisi…[et al.]. 2015. Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056648
Modern Language Association (MLA)
Yan, Fei…[et al.]. Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056648
American Medical Association (AMA)
Yan, Fei& Yang, Wei& Li, Xiang& Liu, Hongmei& Nan, Xiang& Xie, Lisi…[et al.]. Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056648
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056648