American Ginseng Regulates Gene Expression to Protect against Premature Ovarian Failure in Rats
Joint Authors
Zhu, Lei
Li, Ji
Xing, Nannan
Han, Dongwei
Kuang, Haixue
Ge, Pengling
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Premature ovarian failure (POF) is defined as lost ovarian functions before the age of 40.
Three possible molecular markers (PLA2G4A, miR-29a, and miR-144) have been identified in our previous study by integrated analysis of mRNA and miRNA expression profiles.
The present study aimed to evaluate American ginseng root’s protective potential against POF by studying transcriptional and protein variations between American ginseng treatments and controls in rats.
4-Vinylcyclohexene diepoxide (VCD) was administered to rats for 14 days to induce POF.
Additionally, American ginseng was administered to POF rats for one month, and PLA2G4A, miR-29a, and miR-144 expressions were measured in rat ovaries by qRT-PCR.
PLA2G4A protein expression was examined by Western Blot, and PGE2, LH, FSH, and E2 serum levels were detected by ELISA.
PLA2G4A mRNA and protein were downregulated in American ginseng-treated rats, miR-29a and miR-144 levels increased, and PGE2 serum levels decreased, while LH, FSH, and E2 increased compared to POF induction alone.
Analysis of transcriptional and protein variations suggested that American ginseng protects the ovary against POF by regulating prostaglandin biosynthesis, ovulation, and preventing ovarian aging.
High hormone levels (PGE2, FSH, and LH) were reduced, and E2 secretion approached normal levels, leading to improved POF symptoms and abnormal ovulation.
American Psychological Association (APA)
Zhu, Lei& Li, Ji& Xing, Nannan& Han, Dongwei& Kuang, Haixue& Ge, Pengling. 2015. American Ginseng Regulates Gene Expression to Protect against Premature Ovarian Failure in Rats. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056681
Modern Language Association (MLA)
Zhu, Lei…[et al.]. American Ginseng Regulates Gene Expression to Protect against Premature Ovarian Failure in Rats. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056681
American Medical Association (AMA)
Zhu, Lei& Li, Ji& Xing, Nannan& Han, Dongwei& Kuang, Haixue& Ge, Pengling. American Ginseng Regulates Gene Expression to Protect against Premature Ovarian Failure in Rats. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056681
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056681