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Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb3T3 Embryonic Fibroblast Cell Line
Joint Authors
Basińska, Katarzyna
Śmieszek, Agnieszka
Siudzińska, Anna
Marędziak, Monika
Czyrek, Aleksandra
Trynda, Justyna
Skaradzińska, Aneta
Marycz, Krzysztof
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-30
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Metformin, a popular drug used to treat diabetes, has recently gained attention as a potentially useful therapeutic agent for treating cancer.
In our research metformin was added to in vitro cultures of bone marrow-derived multipotent mesenchymal stromal cells (BMSCs) and Balb/3T3 fibroblast at concentration of 1 mM, 5 mM, and 10 mM.
Obtained results indicated that metformin negatively affected proliferation activity of investigated cells.
The drug triggered the formation of autophagosomes and apoptotic bodies in all tested cultures.
Additionally, we focused on determination of expression of genes involved in insulin-like growth factor 2 (IGF2) signaling pathway.
The most striking finding was that the mRNA level of IGF2 was constant in both BMSCs and Balb/3T3.
Further, the analysis of IGF2 concentration in cell supernatants showed that it decreased in BMSC cultures after 5 and 10 mM metformin treatments.
In case of Balb/3T3 the concentration of IGF2 in culture supernatants decreased after 1 and 5 mM and increased after 10 mM of metformin.
Our results suggest that metformin influences the cytophysiology of somatic cells in a dose- and time-dependent manner causing inhibition of proliferation and abnormalities of their morphology and ultrastructure.
American Psychological Association (APA)
Śmieszek, Agnieszka& Czyrek, Aleksandra& Basińska, Katarzyna& Trynda, Justyna& Skaradzińska, Aneta& Siudzińska, Anna…[et al.]. 2015. Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb3T3 Embryonic Fibroblast Cell Line. BioMed Research International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1056686
Modern Language Association (MLA)
Śmieszek, Agnieszka…[et al.]. Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb3T3 Embryonic Fibroblast Cell Line. BioMed Research International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1056686
American Medical Association (AMA)
Śmieszek, Agnieszka& Czyrek, Aleksandra& Basińska, Katarzyna& Trynda, Justyna& Skaradzińska, Aneta& Siudzińska, Anna…[et al.]. Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb3T3 Embryonic Fibroblast Cell Line. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1056686
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056686