Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition
Joint Authors
Wei, Xiao-Xing
Zheng, Wei-Tao
Hou, Xue
Liang, Jian
Li, Zheng-Jun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-07
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The alcohol dehydrogenase promoter P adhE and mixed acid fermentation pathway deficient mutants of Escherichia coli were employed to produce poly(3-hydroxybutyrate) (P3HB) under microaerobic condition.
The E.
coli mutant with ackA-pta, poxB, ldhA, and adhE deletions accumulated 0.67 g/L P3HB, up to 78.84% of cell dry weight in tube cultivation.
The deletion of pyruvate formate-lyase gene pflB drastically decreased P3HB production and P3HB content to 0.09 g/L and 24.44%, respectively.
Overexpressing pflB via the plasmid in its knocked out mutant restored cell growth and P3HB accumulation, indicating the importance of the pyruvate formate-lyase in microaerobic carbon metabolism.
The engineered E.
coli BWapld (pWYC09) produced 5.00 g/L P3HB from 16.50 g/L glucose in 24 h batch fermentation, and P3HB production yield from glucose was 0.30 g/g, which reached up to 63% of maximal theoretical yield.
American Psychological Association (APA)
Wei, Xiao-Xing& Zheng, Wei-Tao& Hou, Xue& Liang, Jian& Li, Zheng-Jun. 2015. Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition. BioMed Research International،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1056726
Modern Language Association (MLA)
Wei, Xiao-Xing…[et al.]. Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition. BioMed Research International No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1056726
American Medical Association (AMA)
Wei, Xiao-Xing& Zheng, Wei-Tao& Hou, Xue& Liang, Jian& Li, Zheng-Jun. Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1056726
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056726