![](/images/graphics-bg.png)
Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four “Kidney-Tonifying” Traditional Chinese Herbs
Joint Authors
Cai, Bin
Zhang, Ai-guo
Zhang, Xian
Ge, Wen-jie
Dai, Guo-da
Tan, Xiang-ling
Roodrajeetsing, Gopaul
Cai, Jian-ping
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Traditional Chinese medicine can promote the proliferation of bone marrow-derived mesenchymal stem cells (BMSCs).
We chose four “Kidney-tonifying” Chinese herbal medicines, Radix Astragali, Salvia, Herba Epimedii, and Saussurea Involucrata, to evaluate whether they had positive effects on the proliferation of BMSCs and TGF-β1-induced chondrogenic differentiation of BMSCs.
The four Chinese herbal medicines were intragastrically administered to Sprague-Dawley rats, respectively, to prepare drug-containing serums of corresponding Chinese herbs.
BMSCs were isolated, cultured, and exposed to culture solution containing 1%, 5%, 10%, and 15% (v/v) Radix Astragali-, Salvia-, Herba Epimedii-, and Saussurea Involucrata-containing serum, respectively.
TGF-β1-induced BMSCs were addressed in the same manner.
Collagen type II protein was assessed by immunofluorescence methods.
To assess whether the drug-containing serums had positive effects on the proliferation of BMSCs and TGF-β1-induced BMSCs, MTT method was assessed.
The proliferation of BMSCs was significantly enhanced when exposed to culture solutions containing 1% and 5% Radix Astragali-, 1% and 5% Salvia-, 5% Herba Epimedii-, and 1%, 5%, and 10% Saussurea Involucrata-containing serum.
The proliferation of TGF-β1-induced BMSCs was significantly enhanced when exposed to 1%, 5%, and 15% Radix Astragali-, 10% and 15% Salvia-, 5%, and 15% Herba Epimedii-, and 1%, 5%, and 10% Saussurea Involucrata-containing serum.
American Psychological Association (APA)
Cai, Bin& Zhang, Ai-guo& Zhang, Xian& Ge, Wen-jie& Dai, Guo-da& Tan, Xiang-ling…[et al.]. 2015. Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four “Kidney-Tonifying” Traditional Chinese Herbs. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056739
Modern Language Association (MLA)
Cai, Bin…[et al.]. Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four “Kidney-Tonifying” Traditional Chinese Herbs. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056739
American Medical Association (AMA)
Cai, Bin& Zhang, Ai-guo& Zhang, Xian& Ge, Wen-jie& Dai, Guo-da& Tan, Xiang-ling…[et al.]. Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four “Kidney-Tonifying” Traditional Chinese Herbs. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056739
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056739