Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress

Abstract EN

LpGPAT was obtained from L.

pensylvanicum using RT-PCR and rapid amplification of cDNA ends.

The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46 KDa.

The nucleic acid sequence analysis showed that it shared high homology with other known GPATs.

SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity.

Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.