The Kampo Medicine Yokukansan Decreases MicroRNA-18 Expression and Recovers Glucocorticoid Receptors Protein Expression in the Hypothalamus of Stressed Mice
Joint Authors
Miyata, Shingo
Shimizu, Shoko
Tohyama, Masaya
Tanaka, Takashi
Takeda, Takashi
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
It is well known that glucocorticoid receptor (GR) signaling regulates the hypothalamic-pituitary-adrenal (HPA) axis, and GR expression level is associated with HPA axis activity.
Recent studies revealed that microRNA- (miR-) 18 and/or 124a are candidate negative regulators of GR in the brain.
The Kampo medicine Yokukansan (YKS) can affect psychological symptoms such as depression and anxiety that are associated with stress responses.
In this study, we evaluated the effect of YKS on miR-18 and 124a and GR levels in mice exposed to stress.
We found that YKS pretreatment normalized elevated plasma corticosterone levels in stress-exposed mice.
In addition, GR mRNA levels were downregulated in the brain following stress exposure.
While miR-124a expression levels were not altered in the hypothalamus of stress-exposed mice, miR-18 levels decreased in the hypothalamus of YKS-pretreated mice after stress exposure.
Finally, GR protein levels in the paraventricular nucleus (PVN) of the hypothalamus after stress exposure recovered in YKS-pretreated mice.
Collectively, these data suggest that YKS normalizes GR protein levels by regulating miR-18 expression in the hypothalamus, thus normalizing HPA axis activity following stress exposure.
American Psychological Association (APA)
Shimizu, Shoko& Tanaka, Takashi& Takeda, Takashi& Tohyama, Masaya& Miyata, Shingo. 2015. The Kampo Medicine Yokukansan Decreases MicroRNA-18 Expression and Recovers Glucocorticoid Receptors Protein Expression in the Hypothalamus of Stressed Mice. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056762
Modern Language Association (MLA)
Shimizu, Shoko…[et al.]. The Kampo Medicine Yokukansan Decreases MicroRNA-18 Expression and Recovers Glucocorticoid Receptors Protein Expression in the Hypothalamus of Stressed Mice. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056762
American Medical Association (AMA)
Shimizu, Shoko& Tanaka, Takashi& Takeda, Takashi& Tohyama, Masaya& Miyata, Shingo. The Kampo Medicine Yokukansan Decreases MicroRNA-18 Expression and Recovers Glucocorticoid Receptors Protein Expression in the Hypothalamus of Stressed Mice. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056762
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056762