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N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells
Joint Authors
Huang, Pei-Rong
Hung, Shu-Chen
Pao, Chia-Chu
Wang, Tzu-Chien V.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
N-(1-pyrenyl) maleimide (NPM) is a fluorescent reagent that is frequently used as a derivatization agent for the detection of thio-containing compounds.
NPM has been shown to display a great differential cytotoxicity against hematopoietic cancer cells.
In this study, the molecular mechanism by which NPM induces apoptosis was examined.
Here, we show that treatment of Jurkat cells with NPM leads to Bak oligomerization, loss of mitochondrial membrane potential (Δψm), and release of cytochrome C from mitochondria to cytosol.
Induction of Bak oligomerization appears to play a critical role in NPM-induced apoptosis, as downregulation of Bak by shRNA significantly prevented NPM-induced apoptosis.
Inhibition of caspase 8 by Z-IETD-FMK and/or depletion of Bid did not affect NPM-induced oligomerization of Bak.
Taken together, these results suggest that NPM-induced apoptosis is mediated through a pathway that is independent of caspase-8 activation.
American Psychological Association (APA)
Huang, Pei-Rong& Hung, Shu-Chen& Pao, Chia-Chu& Wang, Tzu-Chien V.. 2015. N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056769
Modern Language Association (MLA)
Huang, Pei-Rong…[et al.]. N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056769
American Medical Association (AMA)
Huang, Pei-Rong& Hung, Shu-Chen& Pao, Chia-Chu& Wang, Tzu-Chien V.. N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056769
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056769