Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis
Joint Authors
Chen, Jiau-Hua
Chi, Meng-Chun
Lin, Min-Guan
Lin, Long-Liu
Wang, Tzu-Fan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The influence of three sugar osmolytes on the refolding of guanidine hydrochloride- (GdnHCl-) denatured trehalose-6-phosphate hydrolase of Bacillus licheniformis (BlTreA) was studied by circular dichroism (CD) spectra, fluorescence emission spectra, and the recovery of enzymatic activity.
These experimental results clearly indicated that sorbitol, sucrose, and trehalose at a concentration of 0.75 M improved the refolding yields of GdnHCl-denatured BlTreA, probably due to the fact that these sugars favored the formation of tertiary architectures.
Far-UV CD measurements demonstrated the ability of sugar osmolytes to shift the secondary structure of GdnHCl-denatured enzyme towards near-native conformations.
ANS fluorescence intensity measurements revealed a reduction of exposed hydrophobic surfaces upon the treatment of denatured enzyme with sugar osmolytes.
These observations suggest that sugar osmolytes possibly play a chaperone role in the refolding of chemically denatured BlTreA.
American Psychological Association (APA)
Chen, Jiau-Hua& Chi, Meng-Chun& Lin, Min-Guan& Lin, Long-Liu& Wang, Tzu-Fan. 2015. Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056795
Modern Language Association (MLA)
Chen, Jiau-Hua…[et al.]. Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056795
American Medical Association (AMA)
Chen, Jiau-Hua& Chi, Meng-Chun& Lin, Min-Guan& Lin, Long-Liu& Wang, Tzu-Fan. Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056795
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056795