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Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1 β as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients
Joint Authors
Szaflik, Jacek P.
Majsterek, Ireneusz
Pytel, Dariusz
Szymanek, Katarzyna
Markiewicz, Lukasz
Mucha, Bartosz
Szaflik, Jerzy
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of presented work was to analyze the impact of particular polymorphic changes in the promoter regions of the -1607 1G/2G MMP1, -1562 C/T MMP9, -82 A/G MMP12, -511 C/T IL-1β, and 372 T/C TIMP1 genes on their expression level in POAG patients.
Blood and aqueous humor samples acquired from 50 patients with POAG and 50 control subjects were used for QPCR and protein levels analysis by ELISA.
In vivo promoter activity assays were carried on HTM cells using dual luciferase assay.
All studied subjects underwent ophthalmic examination, including BCVA, intraocular pressure, slit-lamp examination, gonioscopy, HRT, and OCT scans.
Patients with POAG are characterized by an increased mRNA expression of MMP1, MMP9, MMP12, and IL-1β genes as compared to the control group ( P < 0.001 ).
Aqueous humor acquired from patients with POAG displayed increased protein expression of MMP1, MMP9, MMP12, and IL-1 β compared to the control group ( P < 0.001 ).
Allele -1607 1G of MMP1 gene possesses only 42,91% of the -1607 2G allele transcriptional activity and allele -1562 C of MMP9 gene possesses only 21,86% of the -1562 T allele.
Increased expression levels of metalloproteinases can be considered as a risk factor for the development of POAG.
American Psychological Association (APA)
Markiewicz, Lukasz& Pytel, Dariusz& Mucha, Bartosz& Szymanek, Katarzyna& Szaflik, Jerzy& Szaflik, Jacek P.…[et al.]. 2015. Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1 β as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056820
Modern Language Association (MLA)
Markiewicz, Lukasz…[et al.]. Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1 β as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056820
American Medical Association (AMA)
Markiewicz, Lukasz& Pytel, Dariusz& Mucha, Bartosz& Szymanek, Katarzyna& Szaflik, Jerzy& Szaflik, Jacek P.…[et al.]. Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1 β as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056820