Predicting Homogeneous Pilus Structure from Monomeric Data and Sparse Constraints
Joint Authors
Sun, Xiao
Xiao, Ke
Shu, Chuanjun
Yan, Qin
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Type IV pili (T4P) and T2SS (Type II Secretion System) pseudopili are filaments extending beyond microbial surfaces, comprising homologous subunits called “pilins.” In this paper, we presented a new approach to predict pseudo atomic models of pili combining ambiguous symmetric constraints with sparse distance information obtained from experiments and based neither on electronic microscope (EM) maps nor on accurate a priori symmetric details.
The approach was validated by the reconstruction of the gonococcal (GC) pilus from Neisseria gonorrhoeae, the type IVb toxin-coregulated pilus (TCP) from Vibrio cholerae, and pseudopilus of the pullulanase T2SS (the PulG pilus) from Klebsiella oxytoca.
In addition, analyses of computational errors showed that subunits should be treated cautiously, as they are slightly flexible and not strictly rigid bodies.
A global sampling in a wider range was also implemented and implied that a pilus might have more than one but fewer than many possible intact conformations.
American Psychological Association (APA)
Xiao, Ke& Shu, Chuanjun& Yan, Qin& Sun, Xiao. 2015. Predicting Homogeneous Pilus Structure from Monomeric Data and Sparse Constraints. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056843
Modern Language Association (MLA)
Xiao, Ke…[et al.]. Predicting Homogeneous Pilus Structure from Monomeric Data and Sparse Constraints. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1056843
American Medical Association (AMA)
Xiao, Ke& Shu, Chuanjun& Yan, Qin& Sun, Xiao. Predicting Homogeneous Pilus Structure from Monomeric Data and Sparse Constraints. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056843