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How to Quantify Penile Corpus Cavernosum Structures with Histomorphometry: Comparison of Two Methods
Joint Authors
Sampaio, Francisco
Felix-Patrício, Bruno
De Souza, Diogo Benchimol
Gregório, Bianca Martins
Costa, Waldemar Silva
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The use of morphometrical tools in biomedical research permits the accurate comparison of specimens subjected to different conditions, and the surface density of structures is commonly used for this purpose.
The traditional point-counting method is reliable but time-consuming, with computer-aided methods being proposed as an alternative.
The aim of this study was to compare the surface density data of penile corpus cavernosum trabecular smooth muscle in different groups of rats, measured by two observers using the point-counting or color-based segmentation method.
Ten normotensive and 10 hypertensive male rats were used in this study.
Rat penises were processed to obtain smooth muscle immunostained histological slices and photomicrographs captured for analysis.
The smooth muscle surface density was measured in both groups by two different observers by the point-counting method and by the color-based segmentation method.
Hypertensive rats showed an increase in smooth muscle surface density by the two methods, and no difference was found between the results of the two observers.
However, surface density values were higher by the point-counting method.
The use of either method did not influence the final interpretation of the results, and both proved to have adequate reproducibility.
However, as differences were found between the two methods, results obtained by either method should not be compared.
American Psychological Association (APA)
Felix-Patrício, Bruno& De Souza, Diogo Benchimol& Gregório, Bianca Martins& Costa, Waldemar Silva& Sampaio, Francisco. 2015. How to Quantify Penile Corpus Cavernosum Structures with Histomorphometry: Comparison of Two Methods. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1056919
Modern Language Association (MLA)
Felix-Patrício, Bruno…[et al.]. How to Quantify Penile Corpus Cavernosum Structures with Histomorphometry: Comparison of Two Methods. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1056919
American Medical Association (AMA)
Felix-Patrício, Bruno& De Souza, Diogo Benchimol& Gregório, Bianca Martins& Costa, Waldemar Silva& Sampaio, Francisco. How to Quantify Penile Corpus Cavernosum Structures with Histomorphometry: Comparison of Two Methods. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1056919
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056919