Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers
Joint Authors
Baccarelli, Andrea A.
Hoet, Peter H. M.
Godderis, Lode
Schouteden, Caroline
Tabish, Ali
Poels, Katrien
Van Landuyt, Kirsten
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aims.
We describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify and compare simultaneously global methylation and hydroxymethylation in human DNA of different tissues.
Materials and Methods.
Blood and saliva DNA from fourteen volunteers was processed for epigenetic endpoints using LC-MS/MS and PCR-pyrosequencing technology.
Results.
Global DNA methylation was significantly lower in saliva (mean 4.61% ± 0.80%), compared to blood samples (5.70% ± 0.22%).
In contrast, saliva (0.036% ± 0.011%) revealed significantly higher hydroxymethylation compared to blood samples (mean 0.027% ± 0.004%).
Whereas we did not find significant correlations for both epigenetic measures between the tissues, a significant association was observed between global methylation and global hydroxymethylation in saliva DNA.
Neither LINE-1 nor Alu elements of blood and saliva correlated, nor were they correlated with the DNA hydroxymethylation of blood or saliva, respectively.
Conclusion.
Global DNA methylation and hydroxymethylation of cytosine can be quantified simultaneously by LC-MS/MS.
Saliva DNA cannot be considered as a surrogate for blood DNA to study epigenetic endpoints.
American Psychological Association (APA)
Godderis, Lode& Schouteden, Caroline& Tabish, Ali& Poels, Katrien& Hoet, Peter H. M.& Baccarelli, Andrea A.…[et al.]. 2015. Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056968
Modern Language Association (MLA)
Godderis, Lode…[et al.]. Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056968
American Medical Association (AMA)
Godderis, Lode& Schouteden, Caroline& Tabish, Ali& Poels, Katrien& Hoet, Peter H. M.& Baccarelli, Andrea A.…[et al.]. Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056968
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056968