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Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens
Joint Authors
Mediavilla-Gradolph, María Concepción
De Toro-Peinado, Inmaculada
Bermúdez-Ruiz, María Pilar
García-Martínez, María de los Ángeles
Ortega-Torres, María
Montiel Quezel-Guerraz, Natalia
Palop-Borrás, Begoña
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species).
A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study.
They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT.
Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M.
avium, M.
intracellulare, M.
abscessus, M.
chelonae, M.
fortuitum, M.
mucogenicum, M.
kansasii, and M.
scrofulaceum).
MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.
American Psychological Association (APA)
Mediavilla-Gradolph, María Concepción& De Toro-Peinado, Inmaculada& Bermúdez-Ruiz, María Pilar& García-Martínez, María de los Ángeles& Ortega-Torres, María& Montiel Quezel-Guerraz, Natalia…[et al.]. 2015. Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1057018
Modern Language Association (MLA)
Mediavilla-Gradolph, María Concepción…[et al.]. Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1057018
American Medical Association (AMA)
Mediavilla-Gradolph, María Concepción& De Toro-Peinado, Inmaculada& Bermúdez-Ruiz, María Pilar& García-Martínez, María de los Ángeles& Ortega-Torres, María& Montiel Quezel-Guerraz, Natalia…[et al.]. Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1057018
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057018