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The Protective Effect of Melatonin on Neural Stem Cell against LPS-Induced Inflammation
Joint Authors
Kang, So Mang
Lee, Kyoung Min
Song, Juhyun
Lee, Jong Eun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-01
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Stem cell therapy for tissue regeneration has several limitations in the fact that transplanted cells could not survive for a long time.
For solving these limitations, many studies have focused on the antioxidants to increase survival rate of neural stem cells (NSCs).
Melatonin, an antioxidant synthesized in the pineal gland, plays multiple roles in various physiological mechanisms.
Melatonin exerts neuroprotective effects in the central nervous system.
To determine the effect of melatonin on NSCs which is in LPS-induced inflammatory stress state, we first investigated nitric oxide (NO) production and cytotoxicity using Griess reagent assays, LDH assay, and neurosphere counting.
Also, we investigated the effect of melatonin on NSCs by measuring the mRNA levels of SOX2, TLX, and FGFR-2.
In addition, western blot analyses were performed to examine the activation of PI3K/Akt/Nrf2 signaling in LPS-treated NSCs.
In the present study, we suggested that melatonin inhibits NO production and protects NSCs against LPS-induced inflammatory stress.
In addition, melatonin promoted the expression of SOX2 and activated the PI3K/Akt/Nrf2 signaling under LPS-induced inflammation condition.
Based on our results, we conclude that melatonin may be an important factor for the survival and proliferation of NSCs in neuroinflammatory diseases.
American Psychological Association (APA)
Song, Juhyun& Kang, So Mang& Lee, Kyoung Min& Lee, Jong Eun. 2015. The Protective Effect of Melatonin on Neural Stem Cell against LPS-Induced Inflammation. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1057019
Modern Language Association (MLA)
Song, Juhyun…[et al.]. The Protective Effect of Melatonin on Neural Stem Cell against LPS-Induced Inflammation. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1057019
American Medical Association (AMA)
Song, Juhyun& Kang, So Mang& Lee, Kyoung Min& Lee, Jong Eun. The Protective Effect of Melatonin on Neural Stem Cell against LPS-Induced Inflammation. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1057019
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057019