Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons
Joint Authors
He, Feng
Wang, Limin
Zhong, Zhuoyuan
Lv, Ruiyan
Xiao, Songhua
Liu, Zhonglin
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Neurotrophic tyrosine kinase type 1 (NTRK1) plays critical roles in proliferation, differentiation, and survival of cholinergic neurons; however, it remains unknown whether enhanced expression of NTRK1 in neural stem cells (NSCs) can promote their differentiation into mature neurons.
In this study, a plasmid encoding the rat NTRK1 gene was constructed and transfected into C17.2 mouse neural stem cells (NSCs).
NTRK1 overexpression in C17.2 cells was confirmed by western blot.
The NSCs overexpressing NTRK1 and the C17.2 NSCs transfected by an empty plasmid vector were treated with or without 100 ng/mL nerve growth factor (NGF) for 7 days.
Expression of the cholinergic cell marker, choline acetyltransferase (ChAT), was detected by florescent immunocytochemistry (ICC).
In the presence of NGF induction, the NSCs overexpressing NTRK1 differentiated into ChAT-immunopositive cells at 3-fold higher than the NSCs transfected by the plasmid vector (26% versus 9%, P < 0.05 ).
The data suggest that elevated NTRK1 expression increases differentiation of NSCs into cholinergic neurons under stimulation of NGF.
The approach also represents an efficient strategy for generation of cholinergic neurons.
American Psychological Association (APA)
Wang, Limin& He, Feng& Zhong, Zhuoyuan& Lv, Ruiyan& Xiao, Songhua& Liu, Zhonglin. 2015. Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1057025
Modern Language Association (MLA)
Wang, Limin…[et al.]. Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1057025
American Medical Association (AMA)
Wang, Limin& He, Feng& Zhong, Zhuoyuan& Lv, Ruiyan& Xiao, Songhua& Liu, Zhonglin. Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1057025
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057025